Fabrication of the artificial tissue by using porous sheets and laser tweezers

为了设计与小组织一样大的细胞簇，我研究了细胞簇形成过程中的细胞信号和脂滴积累。细胞被镀在图案衬底上，可以分别形成2-8个细胞的细胞团。观察到小g蛋白在细胞间连接处活化强烈。此外，我们还观察到，随着单个细胞簇中连接细胞数量的增加，脂滴的积累量也随之增加。

In order to design cell clusters as large as a small tissue, I investigated the cellular signaling and the lipid droplet accumulation depending on the formation of cell clusters. Cells were plated on a pattern substrate so that cell clusters with 2-8 cells could be formed, respectively. It was observed that small G-proteins activated strongly in the joined part between cells. Moreover, it was observed that the amount of accumulated lipid droplets increased with an increase in the number of joined cell in a single cell cluster.