Investigation of factors to enhance anaerobic benzene degradation by ecophysiological approach and application towards remediation of polluted sites

作为一种低成本、低能耗的土壤和地下水中苯污染的处理方法，研究了厌氧苯的降解修复工艺。建立了苯降解菌Hasda-A的定量聚合酶链式反应和荧光原位杂交检测方法。苯的强化降解试验表明，苯的降解总是与Hasda-A的生长相一致的。污染场地地下水的降解以及与氯化乙烯的同时降解也得到了证实。

As a low cost and low energy consumption treatment method for benzene pollution in soil and groundwater, anaerobic benzene degradation was studied to develop remediation process. Detection and quantification method were developed for benzene degrading bacteria Hasda-A by quantitative PCR and Fluorescent in situ hybridization. Enhancement of benzene degradation was tested and the benzene degradation was always coincided with Hasda-A growth. Degradation in groundwater from contaminated sites and simultaneous degradation with chlorinated ethylenes were also demonstrated.