Study of genetic function of the ascidian based on the forward and reverse genetic approaches

基于正向和反向遗传方法的海鞘遗传功能研究

• 批准号: 20681019
• 负责人: SASAKURA Yasunori
• 金额: $ 16.22万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20681019/
• 关键词: 発生・分化; ゲノム; 遺伝子; 突然変異体; トランスポゾン; カタユウレイボヤ; エンハンサートラップ; Minos; UAS-Gal4; Sleeping Beauty; Kaede; 細胞周期; Hox1; 転移酵素; 逆遺伝学; 母性因子; 遺伝子ターゲッティング; Cre-loxP; I-SceI

This study was purposed to establish the methods for investigation of gene functions in the chordate Ciona intestinalis. It was shown that enhancer detection is an effective way to create insertional mutants. It was shown that Hox1 functions in the patterning of epidermis. As a reverse genetic approach, a novel method of targeted disruption of maternally expressed genes was established. Transgenic maker lines were established that express reporter genes in the tissue-specific manner. The neural tissue-specific lines were utilized to investigate how the larval central nervous system was remodeled to construct the adult nervous system during metamorphosis. A method to live-image the cell cycle progression was introduced, and by using this method mechanisms of the coordination between cell division and morphogenesis were shown. A new method of germline transformation in Ciona mediated by Sleeping Beauty transposon was established.

本研究旨在建立研究脊索动物玻璃海鞘基因功能的方法。增强子检测是产生插入突变体的有效方法。研究表明，Hox 1在表皮的图案化中起作用。作为一种反向遗传学方法，建立了一种新的母系表达基因的靶向破坏方法。建立了以组织特异性方式表达报告基因的转基因标记系。利用神经组织特异性线研究了变态过程中幼虫中枢神经系统是如何被改造成成虫神经系统的。介绍了一种实时成像细胞周期进程的方法，并利用该方法揭示了细胞分裂与形态发生之间的协调机制。建立了睡美人转座子介导的玻璃海鞘胚系转化新方法。

项目成果

Efficient Transposition of a Single Minos Transposon Copy in the Genome of the Ascidian Ciona intestinalis With a Transgenic Line Expressing Transposase in Eggs

• DOI: 10.1002/dvdy.22254
• 发表时间: 2010-04-01
• 期刊: DEVELOPMENTAL DYNAMICS
• 影响因子: 2.5
• 作者: Hozumi, Akiko;Kawai, Narudo;Sasakura, Yasunori
• 通讯作者: Sasakura, Yasunori

Developmental changes of nervous system during metamorphosis in the ascidian Ciona intestinalis

海鞘变态过程中神经系统的发育变化

• 发表时间: 2008
• 作者: N.Mori;K.Toume;M.A.Arai;T.Koyano;T.Kowithayakorn;M.Ishibashi;Takeo Horie;M.A.Arai;笹倉靖徳;M.A.Arai;荒井緑;笹倉靖徳;荒井緑(代表);堀江健生
• 通讯作者: 堀江健生

Regeneration of oral siphon pigment organs in the ascidian Ciona intestinalis.

• DOI: 10.1016/j.ydbio.2009.12.040
• 发表时间: 2010-03-15
• 期刊: Developmental biology
• 影响因子: 2.7
• 作者: Auger H;Sasakura Y;Joly JS;Jeffery WR
• 通讯作者: Jeffery WR

Transposon-mediated enhancer detection reveals the location, morphology and development of the cupular organs, which are putative hydrodynamic sensors, in the ascidian Ciona intestinalis.

转座子介导的增强子检测揭示了海鞘中杯状器官的位置、形态和发育，这些器官是假定的流体动力学传感器。

• 发表时间: 2010
• 期刊: Zoological Science
• 影响因子: 0.9
• 作者: M.A.Arai;N.Yanase Maekawa;M.Ishibashi;Naoyuki Ohta
• 通讯作者: Naoyuki Ohta

カタユウレイボヤにおける母性mRNAの特異的ノックダウン技術の開発

海鞘母体mRNA特异性敲低技术的开发

• 发表时间: 2010
• 作者: 飯塚貴子;三田薫;濱田麻友子;佐藤矩行;笹倉靖徳
• 通讯作者: 笹倉靖徳