Comprehensive phenotypic analysis of yeast gene perturbation libraries and development of prediction system

细胞表型如何通过基因修饰改变的问题对于工业用途所需微生物的合理设计是重要的。在此，我们综合分析了酵母菌株在单基因缺失文库和单基因过表达文库中的表型变化。在此基础上，我们发现了对基因扰动具有鲁棒性和脆弱性的基因功能类别，并分析了表达变化与基因扰动响应之间的相关性。

A question how cellular phenotype changes by genetic modifications is important for rational design of desired microorganisms for industrial purpose. Here, we comprehensively analyzed the phynotypic changes of yeast strains in single gene deletion library and single gene overexpression library. Based on the data, we found gene functional categories which are robust or fragile for the gene perturbations, and we also analyzed the correlation between expression changes and response to the gene perturbations.