Proteomic analysis of telencephalin-binding proteins regulating synapse formation

端脑蛋白由端脑内的棘神经元特异性表达，并大量定位于树枝状丝状伪足。我们确定了一种细胞外基质分子，玻连蛋白，作为端脑蛋白的特异性配体。当玻连蛋白包被的珠子被添加到培养的海马神经元，他们坚持树突和强烈诱导端脑蛋白的积累。然后，我们进行了蛋白质组学分析结合玻连蛋白包被珠的神经元复合物。我们确定了几个信号分子定位于树突状丝状伪足。

Telencephalin is specifically expressed by spiny neurons within the telencephalon and abundantly localized to dendritic filopodia. We identified an extracellular matrix molecule, vitronectin, as a specific ligand for telencephalin. When vitronectin-coated beads were added to cultured hippocampal neurons, they adhered onto dendrites and strongly induced telencephalin accumulation. Then, we performed proteomic analysis of neuronal complex bound to vitronectin-coated beads. We determined several signaling molecules localized to dendritic filopodia.