Synthesis and cytotoxicity of anticancer drug intercalated ayered double hydroxide

层状双氢氧化物(LDH)是一类纳米材料，由于其在药物和生物分子传递方面的潜在用途而引起了人们的极大关注。本研究对抗癌药物5-氟尿嘧啶(5-FU)/LDH的合成及其生物大分子(生物分子/5-FU/LDH)的修饰进行了研究。此外，还研究了两种乳酸脱氢酶对多种哺乳动物细胞的细胞毒作用。用X射线衍射仪、傅立叶变换红外光谱等技术对5-FU/LDH进行了表征，证实了5-FU成功插层到LDH中。然后用生物分子对5-FU/LDH进行表面修饰。修饰后的5-FU/LDH保持了原有的LDH结构。5-FU/LDH和生物分子/5-FU/LDH的药效分别是裸5-FU的1.9倍和7.7倍。与5-FU/LDH相比，生物分子/5-FU/LDH具有更快的细胞毒效应。结果表明，生物分子修饰乳酸脱氢酶具有很大的生物相容性药物载体的潜力。

Layered double hydroxide (LDH) is a class of nanomaterials that have attracted considerable attention for their potential utility as drug and biomolecule delivery. In this study, the synthesis of the anticancer drug, (5-fluorouracil (5-FU))/LDH and the modification of the 5-FU/LDH with various biomolecule (biomolecule/5-FU/LDH) have been investigated. Moreover, the cytotoxicity assay of the both LDHs for various mammalian cells has also been studied. The 5-FU/LDH was characterized by various techniques such as XRD and FT-IR that demonstrated the successful intercalation of 5-FU into the LDH. Then, the surface of the 5-FU/LDH was modified with biomolecules. After the modification, the 5-FU/LDH maintained its original LDH structure. The 5-FU/LDH and biomolecule/5-FU/LDH have 1.9 and 7.7 times better drug efficacy than naked 5-FU. The biomolecule/5-FU/LDH exhibited a quick cytotoxicity effect compared with the 5-FU/LDH. The present results demonstrate that the biomolecule modified LDH has the great potential of biocompatible drugcarrier.