Synthesis and cytotoxicity of anticancer drug intercalated ayered double hydroxide

层状双氢氧化物（LDH）是一类纳米材料，因其在药物和生物分子传递方面的潜在用途而受到广泛关注。本研究主要研究了抗癌药物（5-氟尿嘧啶（5-FU））/LDH的合成以及各种生物分子（生物分子/5-FU/LDH）对5-FU/LDH的修饰。此外，还研究了两种低脂多糖对各种哺乳动物细胞的细胞毒性测定。通过XRD和FT-IR等多种技术对5-FU/LDH进行了表征，证实了5-FU成功嵌入LDH。然后，用生物分子修饰5-FU/LDH表面。修饰后，5-FU/LDH保持原有的LDH结构。5-FU/LDH和生物分子/5-FU/LDH的药效分别是裸5-FU的1.9倍和7.7倍。与5-FU/LDH相比，生物分子/5-FU/LDH表现出快速的细胞毒性作用。研究结果表明，生物分子修饰的LDH具有作为生物相容性药物载体的巨大潜力。

Layered double hydroxide (LDH) is a class of nanomaterials that have attracted considerable attention for their potential utility as drug and biomolecule delivery. In this study, the synthesis of the anticancer drug, (5-fluorouracil (5-FU))/LDH and the modification of the 5-FU/LDH with various biomolecule (biomolecule/5-FU/LDH) have been investigated. Moreover, the cytotoxicity assay of the both LDHs for various mammalian cells has also been studied. The 5-FU/LDH was characterized by various techniques such as XRD and FT-IR that demonstrated the successful intercalation of 5-FU into the LDH. Then, the surface of the 5-FU/LDH was modified with biomolecules. After the modification, the 5-FU/LDH maintained its original LDH structure. The 5-FU/LDH and biomolecule/5-FU/LDH have 1.9 and 7.7 times better drug efficacy than naked 5-FU. The biomolecule/5-FU/LDH exhibited a quick cytotoxicity effect compared with the 5-FU/LDH. The present results demonstrate that the biomolecule modified LDH has the great potential of biocompatible drugcarrier.