Development of recovery process for proteins utilizing reversible structural change of stimuli-responsive copolymers

通过变温操作考察了温度敏感型吸附剂对水中牛血清白蛋白(BSA)的吸附和解吸行为。采用N-异丙基丙烯酰胺(NIPA)和乙烯基苄基三甲基氯化铵(VBTA)组成的共聚凝胶和聚(NIPA-co-VBTA)接枝二氧化硅粒子作为温度响应型吸附剂。结果表明，两种共聚物均能在298K时吸附BSA，并在313K时反复脱附部分预吸附的BSA。

The adsorption and desorption behaviors of bovine serum albumin (BSA) in water for the temperature-responsive adsorbents have been investigated by the temperature-swing operation. As the temperature-responsive adsorbents, the copolymer gels composed of N-isopropylacrylamide (NIPA) and vinylbenzyl trimethylammonium chloride (VBTA) and the poly(NIPA-co-VBTA) grafted silica particles were employed. As a result, it was found that both copolymer adsorbents can adsorb BSA at 298 K and desorb some of pre-adsorbed BSA at 313 K repeatedly by the temperature-swing operation.