Establishment of efficient culture systems for somatic stem cells and evaluations of their potentials for articular cartilage disorders

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Autologous chondrocyte implantation is an established technique for the repair of degenerated articular cartilage. However, because of limited properties of the chondrocyte such as senescence after in vitro propagation, application of stem cells are aspired and examined for therapies of some diseases actually. This study aimed to establish efficient method for collection and propagation of the stem cells, and to observe the properties of them as the materials for cell therapy for articular cartilage disorders. As one important result of this study, it has been elucidated that reducing serum concentration in the medium and addition of growth factors is significantly improve the stem cell self-renewal. Furthermore, it has also been demonstrated that this technique could be diverted to the efficient method for induction of mesenchymal progenitor cells, which possess a potential to differentiate to the chondrocytes, from induced pluripotent stem cells.