Genetic analysis of a guanine nucleotide exchanging factor involved in neural tube morphogenesis

参与神经管形态发生的鸟嘌呤核苷酸交换因子的遗传分析

• 批准号: 20890292
• 负责人: TANEGASHIMA Kosuke
• 金额: $ 2.11万
• 依托单位: Tokyo Metropolitan Organization for Medical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20890292/
• 关键词: 先天異常学; 神経管閉鎖障害; 発生・分化; 脳・神経; ノックアウトマウス; WGEF; シグナル伝達; ES細胞; 相同組み替え

Abnormal morphogenesis of the neural tube leads to neural tube defects including spina bifida and anencephaly. We found that a novel guanine nucleotide exchanging factor, WGEF mediates Wnt-PCP pathway, and regulates morphogenic movements of neural tube in the amphibian embryo. In this study, I attempted to generate a conditional knockout mouse of WGEF gene as a mammalian model of neural tube defects. So far, a conditional knockout allele on the WGEF locus was introduced by homologous recombination in the ES cells. Using this ES cells, chimera mice carrying WGEF conditional knockout cells have been generated.

神经管形态发生异常会导致神经管缺陷，包括脊柱裂和无脑畸形。我们发现一种新型鸟嘌呤核苷酸交换因子WGEF介导Wnt-PCP通路，并调节两栖动物胚胎中神经管的形态发生运动。在这项研究中，我试图构建 WGEF 基因条件性敲除小鼠作为神经管缺陷的哺乳动物模型。到目前为止，通过同源重组在 ES 细胞中引入了 WGEF 基因座上的条件敲除等位基因。使用这种 ES 细胞，产生了携带 WGEF 条件敲除细胞的嵌合体小鼠。

项目成果

CXCL14 deficiency attenuates obesity and inhibits feeding behavior in a novel environment

CXCL14 缺乏会减轻肥胖并抑制新环境中的进食行为

• 发表时间: 2009
• 作者: 種子島幸祐;岡本士毅;中山由紀;多屋長治;設楽浩志;石井里絵;米川博通;箕越靖彦;原孝彦
• 通讯作者: 原孝彦

CXCL14 is required for body weight gain in female mice Keystone symposia

雌性小鼠体重增加需要 CXCL14 Keystone 研讨会

• 发表时间: 2009
• 作者: Hara T;Nakayama Y;Tanegashima K
• 通讯作者: Tanegashima K

Coordinated activation of the secretory pathway during notochord formation in the Xenopus embryo

• DOI: 10.1242/dev.036715
• 发表时间: 2009-11
• 期刊: Development
• 影响因子: 4.6
• 作者: Kosuke Tanegashima;Hui Zhao;M. Rebbert;I. Dawid

ケモカインCXCL14の生理的役割とシグナル伝達経路の解析

趋化因子CXCL14的生理作用及信号转导通路分析

• 发表时间: 2008
• 作者: 種子島幸祐;鈴木健司;原孝彦
• 通讯作者: 原孝彦

Antibody-assisted enhancement of biological activities of CXCL14 in human monocytic leukemia-derived THP-1 cells and high fat diet-induced obese mice.

• DOI: 10.1016/j.yexcr.2010.01.016
• 发表时间: 2010-04
• 期刊: Experimental cell research
• 影响因子: 3.7
• 作者: Kosuke Tanegashima;Kenji Suzuki;Y. Nakayama;T. Hara