Role of Saposin B in the intestinal absorption of coenzyme Q10.

我们以前报道过saposin B结合辅酶Q10，使这种脂质溶于水。为了阐明saposin B在肠道中的作用，我们使用Caco-2细胞系。鞘脂激活蛋白B由其前体蛋白鞘脂激活蛋白原产生。我们建立了saposin原基因敲低的caco-2细胞系。saposin原基因敲低的caco-2细胞中辅酶Q10含量低于对照细胞。此外，saposin原敲低导致跨上皮阻力的丧失和紧密连接的破坏。

We previously reported that saposin B binds coenzyme Q10 and makes this lipid soluble in water. In order to clarify the role of saposin B in intestine, we used Caco-2 cell line. Saposin B is produced from its precursor protein, prosaposin. We produced prosaposin knockdown caco-2 cell line. Coenzyme Q10 contents in prosaposin knockdown caco-2 cell line was lower than that in control cell line. Furthermore, prosaposin knockdown resulted in the loss of transepithelial resistance and disruption of tight junctions.