Analyses on Diversity and Functions of Anaerobic Acetate Metabolizing Syntrophic Microorganisms by Using a Liquid Separation and Cultivation Technique.

采用微孔板液体培养法，建立了一种高通量分离培养厌氧乙酸代谢微生物的技术，该微生物包括乙酸产甲烷菌、乙酸氧化菌和氢营养产甲烷菌的微生物共养菌。通过PCR检测培养后微孔板威尔斯孔中的古菌，证实古菌细胞的分布符合泊松分布。利用该技术可以成功地分离和培养厌氧乙酸代谢微生物。

A high-throughput separation and cultivation technique of anaerobic acetate-metabolizing microorganisms, which contain aceticlastic methanogens and microbial syntroph of acetate-oxidizing bacteria and hydrogenotrophic methanogens, was developed by liquid cultivation using microplates. By PCR detection of Archaea in the wells of microplates after cultivation, distribution of archaeal cells was proven to be agreed with Poisson's distribution. The separation and cultivation of anaerobic acetate-metabolizing microorganisms could be successfully carried out using this new technique.