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Analysis on Inter-Cell Electron Transport Systems of Microbial Community in Anaerobic Bioreactor

厌氧生物反应器中微生物群落细胞间电子传递系统分析

基本信息

批准号：
16510061
负责人：
SHIGEMATSU Toru
金额：
$ 2.5万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Under methanogenic conditions, complex organic matters are degraded in a series metabolism by a number of microorganisms of different metabolic abilities. There are several reaction steps, where syntrophic associations between Bacteria and Arcahea are involved. The mechanism of these syntrophic associations has been partly explained based on interspecies electron transport system, although this system remains unknown in detail. In this study we constructed mesophilic anaerobic chemostat cultivations of enriched methanogenic consortia degrading (i) acetate, (ii) propionate or (iii) bovine serum albmin (BSA). Using these chemostats, we analyzed the microbial community structures to understand the syntrophic associations for degrading these substrates and the interspecies electron transport system.Acetate conversion pathway was analyzed using RT-PCR and ^<13>C-labeled acetates. Syntrophic oxidation by acetate oxidizing syntrophs belonging to Firmicutes and hydrogenotrophic methanogens Methanoculleus was suggested to occupy a primary pathway in total methanogenesis at the low dilution rate of 0.025 d^<-1>. In the propionate degradation chemostat, Syntrophobacter relatives and Pelotomaculum relatives were suggested to syntrophically associate with Methanoculleus and/or Methanospirillum and to play the central roles for propionate degradation at the low dilution rate of 0.01 d^<-1> and the high dilution rate of 0.3 d^<-1>, respectively. In the BSA-degrading chemostat, the genus Aminobacterium, was suggested to associate with Methanoculleus and play a central role in amino acid degradation under the condition of Ni^<2+> and Co^<2+> addition, which allowed increased population of methanogenic Archaea.

在产甲烷条件下，复杂的有机物在一系列代谢过程中被许多具有不同代谢能力的微生物降解。有几个反应步骤，其中细菌和古菌之间的联合参与。基于种间电子传递系统，这些共生关联的机制已得到部分解释，尽管该系统的细节尚不清楚。在这项研究中，我们构建了富甲烷菌的中温厌氧趋化培养，降解(i)醋酸酯，（ii）丙酸酯或（iii）牛血清白蛋白（BSA）。利用这些趋化因子，我们分析了微生物群落结构，以了解降解这些底物和种间电子传递系统的共生关系。采用RT-PCR和^<13> c标记的醋酸酯分析醋酸酯转化途径。在0.025 d^<-1>的低稀释率下，厚壁菌门和富氢产甲烷菌（methanouleus）中的醋酸氧化型共养菌的合养氧化被认为是总甲烷生成的主要途径。在丙酸降解趋化因子中，Syntrophobacter亲缘菌和Pelotomaculum亲缘菌与Methanoculleus和/或Methanospirillum共养，分别在低稀释率（0.01 d^<-1>）和高稀释率（0.3 d^<-1>）下对丙酸降解起核心作用。在bsa降解趋化菌中，氨基细菌属与产甲烷菌（Methanoculleus）结合，在添加Ni^<2+>和Co^<2+>的条件下对氨基酸降解起核心作用，使产甲烷古菌数量增加。

项目成果

期刊论文数量（28）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Syntrophism between Bacteria and Archaea of protein and fatty-acids degrading methanogenic consortia. (in Japanese)

蛋白质和脂肪酸降解产甲烷菌群的细菌和古细菌之间的互养性。

DOI：
发表时间：
2004
期刊：
Abstracts of 17th Meeting of Society for Archaea Research, Japan
影响因子：
0
作者：
Y.Suzuki;T.Nakamura;H.Koyama;T.Sekito;Y.Dote;S.Masuda;Toru Shigematsu et al.
通讯作者：
Toru Shigematsu et al.

CO_2固定化・削減・有効利用の最新技術-地球温暖化対策関連技術-

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