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Analysis on the metabolism network of volatile fatty acids by methanogenic consortia

产甲烷菌群挥发性脂肪酸代谢网络分析

基本信息

批准号：
14580593
负责人：
SHIGEMATSU Toru
金额：
$ 2.18万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580593/
关键词：
Methane fermentation methanogenic archaea microbial community gene probe 16S rRNA syntrophic association volatile fatty acids metabolic pathway 16S rRNA遺伝子廃水処理嫌気性処理 mcrA遺伝子 16SrRNA 安定同位体

项目摘要

Acetate conversion pathways of methanogenic consortia in acetate-fed chemostats at dilution rates of 0.025 d^<-1> and 0.6 d^<-1> were investigated using ^<13>C-labeled acetates followed by gas chromatogram mass spectrometry (GC-MS) analysis of CH_4 and CO_2 produced. Non-aceticlastic syntrophic oxidation by acetate oxidizing syntrophs and hydrogenotrophic methanogens was suggested to occupy a primary pathway in total methanogenesis at the low dilution rate. In contrast, aceticlastic cleavage of acetate by aceticlastic methanogens was suggested to occupy a primary pathway in total methanogenesis at the high dilution rate.Phylogenetic analyses of transcripts of the methyl coenzyme M reductase gene (merA) confirmed a significant number of transcripts of the genera Methanoculleus, hydrogenotrophic methanogen, and Methanosarcina, aceticlastic methanogen, were present in the chemostats at the low and high dilution rates, respectively. The mcrA transcripts of the genus Methanosaeta, aceticlas … More tic methanogen, which dominated the population, were poorly detected at the both dilution rate due to the limited diversity coverage of the primers used. These results demonstrated that dilution rate could cause a shift in the primary pathway of acetate conversion to methane in acetate-fed chemostats.The microbial communities of methanogenic consortia in propionate-fed chemostats at dilution rates of 0.01 and 0.3 d^<-1> were investigated using fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE) and 16S rDNA clone analysis. In the domain Archaea, microorganisms closely related to the genera Methanosaeta and Methanoculleus were shown to be predominant at the low dilution rate. In the domain Bacteria, a propionate-oxidizing syntroph Syntrophobacter was shown to be predominant. At the high dilution rate, microorganisms related to the genera Methnaosaeta, Methanoculleus, Methanospirillum were dominant in the domain Archaea. In the domain Bacteria, microorganisms related to the genus Desulfotomaculum were predominant. These results indicated that the microorganisms responsible for degradation of propionate were different by the dilution rates. Less

使用^ 13 C标记的乙酸盐，然后对产生的CH_4和CO_2进行气相色谱质谱(GC-MS)分析，研究了稀释率0.025 d^ -1 > 和0.6 d^ -1 > 的乙酸盐供给恒化器中产甲烷菌群的乙酸盐转化途径。乙酸氧化共养生物和氢营养产甲烷菌的非乙酸共养氧化被认为在低稀释率下占据总产甲烷作用的主要途径。相比之下，醋酸盐产甲烷菌对乙酸盐的醋酸盐裂解被认为在高稀释率下占据总产甲烷作用的主要途径。甲基辅酶M还原酶基因（merA）转录物的系统发育分析证实了Methanoculleus属、氢营养产甲烷菌属和Methanosarcina属的大量转录物，乙酸弹性产甲烷菌分别以低稀释率和高稀释率存在于恒化器中。由于所用引物的多样性覆盖范围有限，在种群中占主导地位的甲烷菌属、醋酸菌属的 mcrA 转录本在两种稀释率下均检测不到。这些结果表明，稀释率可能导致醋酸盐补给恒化器中乙酸盐转化为甲烷的主要途径发生变化。使用荧光原位杂交（FISH）、变性梯度凝胶电泳（DGGE）和16S rDNA克隆对稀释率为0.01和0.3 d^<-1>的丙酸盐补给恒化器中产甲烷菌群的微生物群落进行了研究分析。在古菌领域，与甲烷菌属和甲烷库菌属密切相关的微生物在低稀释率下占主导地位。在细菌领域，丙酸氧化型互养细菌（Syntropobacter）被证明是主要的。在高稀释率下，与 Methnaosaeta、Methanoculleus、Methanospirillum 属相关的微生物在古细菌领域中占优势。在细菌领域，与 Desulfotomaculum 属相关的微生物占主导地位。这些结果表明，负责丙酸盐降解的微生物因稀释率而异。较少的