Cloning of cDNAs encoding enzymes involved in the natural product biosynthesis of leguminous plants using functional expression screening.

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More than 90% of isoflavonoids are present in the leguminous plants. In the leguminous plants, isoflavonoids are typical ecophysiologicallyactive compounds such as defense substances and signal molecules in the early stages of symbiosis with rhizobial bacteria. In this study, we identified several cDNAs encoding enzymes in isoflavonoid pathway by screening of cDNA expression library. A pilot experiment of biotechnological production of isoflavonoids was examined.