Intracellular protein delivery by high pressure modulation

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A novel method for the intracellular delivery of protein using high pressure modulation was developed. The protein transduction domain(PTD), a membrane permeable basic peptide, was conjugated with the antigen model protein ovalbumin(OVA). The heat treatment of the OVA with PTD peptide resulted in the formation of the protein nanoparticle with the zeta potential of-25 mV. On the other hand, the zeta potential of nanoparticle obtained by the pressure treatment at 400 MPa was-15 mV indicating that PTD peptide was localized on the surface of the protein nanoparticle. These results indicate that high pressure modulation is a useful method to improve the the cell uptake of protein nanoparticle.