Biopolymer Functions under High Pressure

生物聚合物在高压下发挥作用

• 批准号: 01470103
• 负责人: KUNUGI Shigeru
• 金额: $ 4.22万
• 依托单位: Kyoto Institute of Technology (1990)University of Fukui (1989)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01470103/
• 关键词: High pressure; Biopolymers; Proteoses; Nucleases; Chemical Modification; Denaturation; Proteins; タンパク質分解酵素; 電子伝達

Pressure is a thermodynamic parameter as important as temperature and lately many efforts are paid to use pressure as a new technique of processing of biopolymers as for the food processing. On a molecular basis pressure has been used to study the refolding and dissociation of proteins. These pressure effects are essentially based on the structural perturbation of mostly proteins caused by increasing pressure. The structural perturbation of the enzymes and proteins by pressure is highly dependent on the protein nature and oligomeric compositions.On the other hand, the pressure effect on enzyme activity is also based on the perturbation on protein structure and protein-ligand interactions, caused by increasing pressure. Sometimes pressure can even affect substrate or reaction specificity of enzymes and such an effect has been uniquely utilized to control some enzyme-catalyzed synthetic reactions by way of pressure.In this study we proceeded this principle to 1, study of the mechanism of enzyme reactions such as nucleases and proteoses ; 2, control of the productive enzyme reactions by pressure and 3, chemical and biochemical modification of an enzyme under reversible denaturation caused by high pressure.

压力是一个和温度一样重要的热力学参数，近年来，压力作为生物聚合物加工的一种新技术，在食品加工中也得到了广泛的应用。在分子基础上，压力已被用来研究蛋白质的折叠和解离。这些压力效应本质上是基于压力增加引起的大部分蛋白质的结构扰动。压力对酶和蛋白质结构的扰动高度依赖于蛋白质的性质和低聚物组成，另一方面，压力对酶活性的影响也基于压力增加对蛋白质结构和蛋白质-配体相互作用的扰动。有时压力甚至会影响酶的底物或反应专一性，这种效应已被独特地用于控制一些酶催化的合成反应。在本研究中，我们将这一原理推广到1，研究核酸酶和蛋白水解酶等酶反应的机理；2，压力控制产生的酶反应；3，高压引起的可逆变性的酶的化学和生化修饰。

项目成果

S. Kunugi, T. Kondoh, H. Hayashiba, A. Nomura: ""Carboxypeptidaset Y-catalyzed Reaction in Systems Containing Organic Solvent"," Biocatalysis. 4. 141-151 (1990)

S. Kunugi、T. Kondoh、H. Hayashiba、A. Nomura：“含有有机溶剂的系统中的羧肽组 Y 催化反应”，生物催化。

功刀 滋: "“Effectof pressure on plant endonuclease reactions"" Nucleic Acids Research. 21. 133-134 (1989)

Shigeru Kuto：“压力对植物核酸内切酶反应的影响”《核酸研究》21. 133-134 (1989)。

林 力丸編,功刀 滋: "加圧食品ー研究と開発(分担分:選択的変性剤としての圧力)" さんえい出版, 374 (1990)

Rikimaru Hayashi（编）、Shigeru Koto（编）：“加压食品 - 研究与开发（共享部分：压力作为选择性变性剂）” Sanei Publishing，374（1990）

S. Kunugi, K. Iwasaki. A. Nomura: ""Effect of pressure on plant endonuclease reactions"" Nucleic Acids Res., Symp. Ser.21. 133-134 (1989)

S. Kunugi，K. 岩崎。

功刀 滋: "“Study on the inactivation of wheat carboxypeptidase.Implication of the transient active monomer and the halfーsite reactivity"." European J.Biochemistry. (1991)

Shigeru Kuto：“小麦羧肽酶失活的研究。瞬时活性单体和半位点反应性的影响”。欧洲生物化学杂志（1991）。