Mechanosensing in vascular endothelial cells and its response to the physiological stress environment

血管内皮细胞的机械传感及其对生理应激环境的响应

• 批准号: 21560195
• 负责人: TADA Shigeru
• 金额: $ 3万
• 依托单位: 防衛大学校
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21560195/
• 关键词: バイオ流体力学; 動脈硬化症; 低比重リポ蛋白; 血管平滑筋細胞; せん断応力; 血管壁伸張; 一酸化窒素; 血管内皮細胞

The present study demonstrated that : I) Exposure of cultured bovine aortic endothelial cells(BAEC) to fluid flow resulted in nitric oxide(NO) production and up-regulation of intracellular calcium ion concentration([Ca2+] i), monitored by fluorescent dyes of DAF2-DA and Rhod2-DA, respectively using a confocal laser scanning microscope, II) A two-dimensional(2-D) numerical simulation of a convective-diffusive transport of LDL in the artery wall coupled with the wall shear stress gradient(WSSG) dependent LDL consumption of smooth muscle cells(SMCs) resulted in a strong dependence of LDL uptake of SMCs on WSSG for cells right beneath the fenestral pores, and III) Construction of a three-dimensional models of bifurcations with two different bifurcation angles mimicking the real bifurcation anatomy to perform simulations of steady blood flow under the wall boundary condition of a constant oxygen concentration, resulted in the significant alternation of the distribution and the magnitude of the oxygen wall flux separation at the outer common-internal carotid wall, depending strongly on the bifurcation angle.

本研究表明：I）将培养的牛主动脉内皮细胞（BAEC）暴露于流体流导致一氧化氮（NO）产生和细胞内钙离子浓度的上调（[Ca 2 +] i），使用共聚焦激光扫描显微镜分别通过DAF 2-DA和Rhod 2-DA的荧光染料监测，II）LDL在动脉壁中的对流-扩散运输与平滑肌细胞（SMC）的壁剪切应力梯度（WSSG）依赖性LDL消耗耦合的二维（2-D）数值模拟导致SMC的LDL摄入对窗孔下方细胞的WSSG有很强的依赖性，（三）建设一个三-模拟真实的分叉解剖结构的具有两个不同分叉角度的分叉的三维模型，以在恒定氧气的壁边界条件下模拟稳态血流的浓度，导致在显着的交替的分布和幅度的氧壁通量分离在外部共同颈内动脉壁，强烈地依赖于分叉角。

项目成果

Computatio-nal study of LDL mass transport in the artery wall

动脉壁中低密度脂蛋白质量转运的计算研究

• DOI: 10.1007/s12573-011-0034-3
• 发表时间: 2011
• 期刊: Journal of Biorheology
• 作者: Shigeru Tada;Hirokazu Ozono
• 通讯作者: Hirokazu Ozono

せん断応力刺激による内皮細胞NO活性機構

剪切应力刺激诱导内皮细胞NO活化的机制

• 发表时间: 2010
• 作者: N. S. Midi;R. Ohyama;久田重善;多田茂
• 通讯作者: 多田茂