Elucidation of flavone C-glycosides biosynthesis pathway in rice based on transcriptome analysis

基于独立开发的相关系数数据库的转录组共表达分析表明，黄酮类c -糖苷生物合成途径中基因的表达模式与查尔酮合成酶和查尔酮异构酶的编码基因相关，提示一些基因可能参与黄酮类c -糖苷生物合成。通过对水稻多个器官的类黄酮分析，检测到29个类黄酮相关峰，并对其中11个峰的结构进行了推测。在部分FOX水稻品系中检测到额外的类黄酮相关峰。通过重组蛋白酶活性测定和在拟南芥中的过表达，以及分子遗传学分析来检测候选基因的功能。

Transcriptome coexpression analysis based on correlation coefficients database developed independently showed that the expression patterns of genes in flavonoid C-glycoside biosynthetic pathway are correlated with genes encoding chalcone synthase and chalcone isomerase and suggests some genes as candidates involved in flavonoid C-glycoside biosynthesis. By flavonoid analyses using several rice organs, 29 flavonoid-related peaks were detected and the structures of 11 peaks were supposed. Additional flavonoid related peaks were detected in some rice FOX lines. The functions of candidate genes were assayed by measurement of enzymatic activity using recombinant proteins and overexpression in Arabidopsis in addition to molecular genetic analysis.