Molecular mechanisms of sexual plasticity during testicular differentiation and development in the medaka, Olyzias latipes

青鳉睾丸分化和发育过程中性可塑性的分子机制

• 批准号: 21570065
• 负责人: KOBAYASHI Tohru
• 金额: $ 3.08万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570065/
• 关键词: メダカ; 性的可塑性; 性分化; 精巣卵; エストロゲン; dmrt1; foxl2; セルトリ細胞; dmrtl; 性転換; 精巣

This study focuses on the molecular mechanisms of sexual plasticity of testicular somatic cells during testis differentiation and development in the medaka, Olyzias latipes. In XY medaka of Hd-rR strain, estrogen treatment induces the female differentiation when estrogen expose to the fry before 30 dph(days post hatching). During XY sex reversal by estrogen, Sertoli cells showed the expression of foxl2, whereas the expression of male differentiation maker genes of germ-cell supporting cells were decreased and subsequently suppressed, such as gsdf and dmrt1 etc. Although estrogen induced testis-ova differentiation easily in mature fish, XY sex reversal(ovarian differentiation) was not induced. In mature XY fish treated by estrogen, foxl2 expression was induced. However, expression of male differentiation maker genes was not suppressed completely. These results suggest strongly that sexual plasticity of Sertoli cells is limited with the progression of testis differentiation in medaka.

本研究重点研究青鳉 Olyzias latipes 睾丸分化和发育过程中睾丸体细胞性可塑性的分子机制。在Hd-rR品系的XY青鳉中，当雌激素在30 dph（孵化后天）之前暴露于鱼苗时，雌激素处理诱导雌性分化。在雌激素诱导的XY性别逆转过程中，支持细胞表达foxl2，而生殖细胞支持细胞的雄性分化标记基因如gsdf和dmrt1等表达减少并随后受到抑制。雌激素虽然容易诱导成熟鱼的睾丸-卵子分化，但不诱导XY性别逆转（卵巢分化）。在用雌激素处理的成熟XY鱼中，诱导foxl2表达。然而，雄性分化标记基因的表达并未被完全抑制。这些结果强烈表明，随着青鳉睾丸分化的进展，支持细胞的性可塑性受到限制。

项目成果

魚類精巣分化過程にお希有r体細胞の挙動

鱼类睾丸分化过程中稀有体细胞的行为

• 发表时间: 2009
• 作者: 三田雅敏;小林亨
• 通讯作者: 小林亨

エストロゲンによるメダカ精巣卵形成誘起過程における精原細胞の動態

雌激素诱导青鳉睾丸卵子发生过程中精原细胞的动态变化

• 发表时间: 2011
• 作者: 吉国通庸;三田雅敏;大野薫;山野恵祐;千葉文香・佐藤忠・井口泰泉・濱口哲・小林亨
• 通讯作者: 千葉文香・佐藤忠・井口泰泉・濱口哲・小林亨

魚類の生殖腺性分化と性的可塑性

鱼类性腺性别分化和性可塑性

• 发表时间: 2011
• 作者: Kobayashi;T.;三田雅敏;小林亨
• 通讯作者: 小林亨

Comparative Responsiveness to test and sentinel fish species to natural and synthetic oestrogens

测试鱼种和哨兵鱼种对天然和合成雌激素的比较反应性

• 发表时间: 2011
• 作者: Lange;A.;Katsu;Y.;Miyagawa;S.;Ogino;Y.;Urushutani;H.;Kobayashi;T.;Hirai;T.;Shears;J. A.;Nagae;M.;Yamamoto;5 J.;Ohnishi;Y.;Oka;T.;Tatarazako;N.;Ohta;Y.;Tyler;C. R. and Iguchi;T.
• 通讯作者: T.

Sexual dimorphisms in somatic cells in the testis of medaka during estrogen-induced testis-ova differentiation.

雌激素诱导睾丸-卵子分化过程中青鳉睾丸体细胞的性别二态性。

• 发表时间: 2012
• 作者: Kobayashi;T.
• 通讯作者: T.