Development of single-molecule tracking using fluorescent silicon nanoparticles and its application to signaling mechanisms in clathrin-coated pits

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Single receptor molecules in the plasma membrane were specifically labeled with fluorescent silicon nanoparticles without blinking or photobleaching, making it possible for the first time to visualize individual internalization events through clathrin-coated pits. A method for conjugating ligands for enzyme-based protein tag to nanoparticle surface was developed, so that the versatility of the probe was enhanced. A new camera system was further developed, allowing us to detect each elementary process of receptor association and signaling complex formation at a 0. 1-ms resolution.