Molecular analysis of anaerobic benzene degradation

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Intermediates formed during anaerobic benzene degradation by Azoarcus sp. DN11 was identified by gas chromatography/mass spectrometry. The data support initial methylation of benzene to toluene, followed by transformation to benzoate. Benzene-induced gene expression analysis suggested that there was a new methyltransferase gene near an anaerobic toluene degradation gene cluster. The overexpressed recombinant methyltransferase converted benzene to a metabolite that has the same retention time with toluene.