Development of a general method for obtaining double KO swine cells which will be used as donors for production of cloned homozygous piglets
Development of a general method for obtaining double KO swine cells which will be used as donors for production of cloned homozygous piglets
批准号:
21580350
负责人:
SATO Masahiro
金额:
$3.16万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
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英文摘要
This study aims to develop a general method for obtaining double KO swine cels in which the target gene expression is completely blocked. This was successfully performed by selection of heterogenous KO cels under higher concentrations of selective drugs after repeated delivery of a targeting vector(RDTV). These resulting double KO cells will be used for somatic cell nuclear transfer-mediated production of cloned pigs.
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Production of genetically modified porcine blastocysts by somatic cell nuclear transfer : preliminary results toward production of xenograft-competent miniature pigs
通过体细胞核移植生产转基因猪囊胚:生产具有异种移植能力的小型猪的初步结果
DOI:
--
发表时间:
2010
期刊:
J Reprod Dev
影响因子:
--
作者:
[Himaki T, Watanabe S, Chi H, Yoshida M, Miyoshi K, Sato M]
通讯作者:
Sato M
DOI:
10.1111/j.1740-0929.2010.00772.x
发表时间:
2010-01-01
期刊:
ANIMAL SCIENCE JOURNAL
影响因子:
2
作者:
[Himaki, Takehiro, Yokomine, Taka-aki, Yoshida, Mitsutoshi]
通讯作者:
Yoshida, Mitsutoshi
DOI:
10.1111/j.1399-3089.2010.00568.x
发表时间:
2010-01-01
期刊:
XENOTRANSPLANTATION
影响因子:
3.9
作者:
[Akasaka, Eri, Watanabe, Satoshi, Sato, Masahiro]
通讯作者:
Sato, Masahiro
Skt^<Cre>系統を用いたコンディショナルノックアウト法による椎間板形成の解析
使用 Skt^<Cre> 菌株通过条件敲除法分析椎间盘形成
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Otani H, 宇田川潤, 大楠美佐子, 首藤政親, 首藤政親, 谷河麻耶]
通讯作者:
谷河麻耶
Successful suppression of endogenous α-1, 3-galactosyltransferase expression by RNA interference in pig embryos generated in vitro
通过 RNA 干扰成功抑制体外猪胚胎中内源 α-1, 3-半乳糖基转移酶的表达
DOI:
--
发表时间:
2012
期刊:
J Reprod Dev
影响因子:
--
作者:
[Chi H, Shinohara M, Yokomine T, Sato M, Takao S, Yoshida M, Miyoshi K.]
通讯作者:
Miyoshi K.
共 28 条
Efficient enrichment of homozygous bi-allelic knockout microminiature porcine cells using a novel selection system and production of genome-edited cloned piglets
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批准号:15K07695
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.24万
-
财政年份:2015
-
负责人:SATO Masahiro
-
依托单位:
Production of cloned genetically modified pigs by a new drug-free gene-modified cell isolation system
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批准号:24580411
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项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.58万
-
财政年份:2012
-
负责人:SATO Masahiro
-
依托单位:
Methods of characterizing magnetic multi-polar and spin-liquid phases
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批准号:21740295
-
项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.66万
-
财政年份:2009
-
负责人:SATO Masahiro
-
依托单位:
Production and analysis of transgenic mice exhibiting concentric hypertrophy in a heart using Cre-loxP system
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批准号:11470165
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$3.26万
-
财政年份:1999
-
负责人:SATO Masahiro
-
依托单位:
Fundamental Investigation of Ultrasonic properties in Biological Tissues by Numerical Analysis.
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批准号:06680869
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项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$1.47万
-
财政年份:1994
-
负责人:SATO Masahiro
-
依托单位:
海外基金