Development of a general method for obtaining double KO swine cells which will be used as donors for production of cloned homozygous piglets

开发获得双 KO 猪细胞的通用方法，该细胞将用作生产克隆纯合仔猪的供体

• 批准号: 21580350
• 负责人: SATO Masahiro
• 金额: $ 3.16万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580350/
• 关键词: KOブタ; ホモ; gene targeting; 薬剤耐性; 体細胞核移植; 胎仔性繊維芽細胞; 遺伝子導入; double KO; ホモKOブタ; gene targetin; ホセKOブタ

This study aims to develop a general method for obtaining double KO swine cels in which the target gene expression is completely blocked. This was successfully performed by selection of heterogenous KO cels under higher concentrations of selective drugs after repeated delivery of a targeting vector(RDTV). These resulting double KO cells will be used for somatic cell nuclear transfer-mediated production of cloned pigs.

本研究旨在建立一种获得目的基因表达完全被阻断的双KO猪源的通用方法。这是通过在重复递送靶向载体（RDTV）后在较高浓度的选择性药物下选择异源KO载体成功进行的。这些产生的双KO细胞将用于体细胞核转移介导的克隆猪生产。

项目成果

Production of genetically modified porcine blastocysts by somatic cell nuclear transfer : preliminary results toward production of xenograft-competent miniature pigs

通过体细胞核移植生产转基因猪囊胚：生产具有异种移植能力的小型猪的初步结果

• 发表时间: 2010
• 期刊: J Reprod Dev
• 作者: Himaki T;Watanabe S;Chi H;Yoshida M;Miyoshi K;Sato M
• 通讯作者: Sato M

Effects of trichostatin A on in vitro development and transgene function in somatic cell nuclear transfer embryos derived from transgenic Clawn miniature pig cells

• DOI: 10.1111/j.1740-0929.2010.00772.x
• 发表时间: 2010-01-01
• 期刊: ANIMAL SCIENCE JOURNAL
• 影响因子: 2
• 作者: Himaki, Takehiro;Yokomine, Taka-aki;Yoshida, Mitsutoshi
• 通讯作者: Yoshida, Mitsutoshi

Enrichment of xenograft-competent genetically modified pig cells using a targeted toxin, isolectin BS-I-B4 conjugate

• DOI: 10.1111/j.1399-3089.2010.00568.x
• 发表时间: 2010-01-01
• 期刊: XENOTRANSPLANTATION
• 影响因子: 3.9
• 作者: Akasaka, Eri;Watanabe, Satoshi;Sato, Masahiro
• 通讯作者: Sato, Masahiro

Skt^<Cre>系統を用いたコンディショナルノックアウト法による椎間板形成の解析

使用 Skt^<Cre> 菌株通过条件敲除法分析椎间盘形成

• 发表时间: 2010
• 作者: Otani H;宇田川潤;大楠美佐子;首藤政親;首藤政親;谷河麻耶
• 通讯作者: 谷河麻耶

Successful suppression of endogenous α-1, 3-galactosyltransferase expression by RNA interference in pig embryos generated in vitro

通过 RNA 干扰成功抑制体外猪胚胎中内源 α-1, 3-半乳糖基转移酶的表达

• 发表时间: 2012
• 期刊: J Reprod Dev
• 作者: Chi H;Shinohara M;Yokomine T;Sato M;Takao S;Yoshida M;Miyoshi K.
• 通讯作者: Miyoshi K.