Exploration of factors that have an influence on reassortment of influenza A virus

影响甲型流感病毒重排的因素探讨

• 批准号: 21580372
• 负责人: GOTO Hideo
• 金额: $ 3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580372/
• 关键词: インフルエンザ; 遺伝子再集合; 断片化蛋白質; コンプリメンテーション; 断片化ルシフェラーゼ

To explore causes that have an influence on reassortment of influenza A virus, virus like particles that carried HA and NA genome segments expressing split reporter proteins were generated. Reporter activity by protein complementation was detectable in cells transfected with protein expression plasmids. However the activity was reduced to 1/105 of the wild type activity. Due to the significant reduction of the activity the complementation could not be detected upon the VLP infection. The different amount of protein production between HA and NA segments may contribute the undetectable complementation upon VLP infection

为了探索影响甲型流感病毒重配的原因，产生了携带表达分裂报告蛋白的HA和NA基因组区段的病毒样颗粒。在用蛋白表达质粒转染的细胞中可检测到通过蛋白互补的报告活性。然而，活性降低至野生型活性的1/105。由于活性的显著降低，在VLP感染后不能检测到互补。HA和NA片段之间蛋白质产生量的不同可能导致VLP感染后无法检测到的互补作用

项目成果

Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNA.

• DOI: 10.1016/j.jviromet.2010.12.014
• 发表时间: 2011-04
• 期刊: JOURNAL OF VIROLOGICAL METHODS
• 影响因子: 3.1
• 作者: Kawakami, Eiryo;Watanabe, Tokiko;Fujii, Ken;Goto, Hideo;Watanabe, Shinji;Noda, Takeshi;Kawaoka, Yoshihiro
• 通讯作者: Kawaoka, Yoshihiro

Sumoylation of influenza A virus nonstructural protein 1 is important for type I interferon antagonism and pathogenicity

甲型流感病毒非结构蛋白 1 的苏酰化对于 I 型干扰素拮抗和致病性很重要

• 发表时间: 2011
• 作者: Kakugawa S;Shimojima M;Neumann G;Goto H;Kawaoka Y;Ozawa M;Konno H;T. Gorai
• 通讯作者: T. Gorai

TRAF6 establishes innate immune responses by activating NF-kappaB and IRF7 upon sensing cytosolic viral RNA and DNA.

• DOI: 10.1371/journal.pone.0005674
• 发表时间: 2009-05-25
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Konno H;Yamamoto T;Yamazaki K;Gohda J;Akiyama T;Semba K;Goto H;Kato A;Yujiri T;Imai T;Kawaguchi Y;Su B;Takeuchi O;Akira S;Tsunetsugu-Yokota Y;Inoue J
• 通讯作者: Inoue J

Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-1

• DOI: 10.1038/nature09420
• 发表时间: 2010-10-14
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Arii, Jun;Goto, Hideo;Kawaguchi, Yasushi
• 通讯作者: Kawaguchi, Yasushi

The HA and NS genes of human H5N1 influenza A virus contribute to high virulence in ferrets.

• DOI: 10.1371/journal.ppat.1001106
• 发表时间: 2010-09-16
• 期刊: PLoS pathogens
• 影响因子: 6.7
• 作者: Imai H;Shinya K;Takano R;Kiso M;Muramoto Y;Sakabe S;Murakami S;Ito M;Yamada S;Le MT;Nidom CA;Sakai-Tagawa Y;Takahashi K;Omori Y;Noda T;Shimojima M;Kakugawa S;Goto H;Iwatsuki-Horimoto K;Horimoto T;Kawaoka Y
• 通讯作者: Kawaoka Y