Investigation of pathophysiologic mechanisms of acute cardiogenic shock by necrotizing toxins from gas gangrene bacilli.

气性坏疽杆菌坏死毒素研究急性心源性休克的病理生理机制。

• 批准号: 21580379
• 负责人: MUKAMOTO Masafumi
• 金额: $ 3.16万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580379/
• 关键词: 悪性水腫; クロストリジウム; 細胞壊死毒素; 心臓; 心不全; セプチカム

Contraction of cultured cardiomyocytes from rat fetus heart ventricles have been stopped by pore formation of alpha-toxin on cell membranes. It was suggested that decrease of ATP productions and desiccation of ATP in cardiomyocytes have been caused by mitochondrial myopathy from Ca^<2+> excess in cytoplasm. It has been clear that tryptophan of 338th and 342th, hydrophobic amino acids of 340th and negative charged amino acids of 339th play an important roles in cell binding and cytotoxic activities of alpha-toxin.

体外培养的大鼠胎儿心室心肌细胞被α -毒素在细胞膜上形成小孔而停止收缩。提示心肌细胞ATP生成减少和ATP干燥是由细胞质中Ca^<2+>过量引起的线粒体肌病引起的。结果表明，第338和342号色氨酸、第340号疏水氨基酸和第339号负电荷氨基酸在α -毒素的细胞结合和细胞毒活性中起重要作用。

项目成果

微生物胞子(渡部一仁、土戸哲明、坂上吉一編)

微生物孢子（渡边一仁、渡渡哲明、坂上吉和编辑）

• 发表时间: 2011
• 作者: Yoshihiro Kaku;Akira Noguchi;Kozue Hotta;Akio Yamada;Satoshi Inoue;向本雅郁
• 通讯作者: 向本雅郁

Characterization of the D/C mosaic neurotoxin produced by Clostridium botulinum associated with bovine botulism in Japan

• DOI: 10.1016/j.vetmic.2009.07.023
• 发表时间: 2010-01-06
• 期刊: VETERINARY MICROBIOLOGY
• 影响因子: 3.3
• 作者: Nakamura, Keiji;Kohda, Tomoko;Kozaki, Shunji
• 通讯作者: Kozaki, Shunji

微生物胞子

微生物孢子

• 发表时间: 2011
• 作者: Kozue Hotta;Hiroki Takakuwa;Le Thi Quynh Mai;Song Phuong Lien;Toshiyuki Murase;Etsuro Ono;Toshihiro Ito;Koichi Otsuki;Tetsu Yamashiro;向本雅郁
• 通讯作者: 向本雅郁