Analysis of intracellular localization of clock protein by means of bioluminescence imaging technique

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To visualize intracellular localization of clock protein by means of bioluminescence imaging technique, we have developed an enhanced green-emitting luciferase(ELuc) probe. ELuc exhibits a light signal in mammalian cells that is over 10-fold stronger than that of the firefly luciferase(FLuc), which is the most widely used luciferase reporter gene. We showed that ELuc produces a strong light signal in primary cells and tissues and that it enables the visualization of gene expression with high temporal resolution at the single-cell level. Moreover, we successfully imaged the nucleocytoplasmic shuttling of clock protein(mPER2 and mCRY2) by fusing ELuc at the intracellular level.