Analysis of myosin sliding on wide variety of actin-rails.

肌球蛋白在各种肌动蛋白轨道上滑动的分析。

• 批准号: 21590270
• 负责人: ISHIKAWA Ryoki
• 金额: $ 3万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590270/
• 关键词: アクチン; ミオシン; アクチン結合タンパク質; アクチン結合蛋白質

To examine whether or not brain-specific actin binding protein drebrin affects the activity of myosin-V, we observed the sliding of myosin-V on F-actin in the presence or absence of drebrin. We found that myosin-V slides on drebrin-decorated F-actin as fast as "naked" F-actin, whereas sliding distance and attachment time were smaller in the presence of drebrin than that in the absence of drebrin. Drebrin inhibits the binding of leading head to F-actin, but does not inhibit the release of ADP from trailing head. These results suggest that drebrin modulates the affinity of myosin-V to F-actin, thus inhibits the activity of myosin-V, and affects the motility of the central zone of nerve cell growth cones.

为了检测脑特异性肌动蛋白结合蛋白dreplastin是否影响肌球蛋白-V的活性，我们观察了在dreplastin存在或不存在的情况下肌球蛋白-V在F-肌动蛋白上的滑动。我们发现肌球蛋白-V在drebrin修饰的F-actin上的滑动速度与“裸”F-actin一样快，而在drebrin存在下的滑动距离和附着时间比在drebrin不存在下的小。Dreglutamine抑制F-肌动蛋白与前导头的结合，但不抑制ADP从尾头的释放。这些结果表明，dreglutamine调节肌球蛋白V与F-肌动蛋白的亲和力，从而抑制肌球蛋白V的活性，并影响神经细胞生长锥中央区的运动。

项目成果

Down-Regulation of Myosin Light Chain Kinase Expression in Vascular Smooth Muscle Cells Accelerates Cell Proliferation: Requirement of Its Actin-binding Domain for Reversion to Normal Rates

血管平滑肌细胞中肌球蛋白轻链激酶表达的下调加速细胞增殖：需要其肌动蛋白结合域才能恢复正常速率

• DOI: 10.1254/jphs.11213sc
• 发表时间: 2012
• 期刊: Journal of Pharmacological Sciences
• 影响因子: 3.5
• 作者: Wang HH;Nakamura A;Yoshiyama S;Ishikawa R;Cai N;Ye LH;Takano-Ohmuro H;Kohama K
• 通讯作者: Kohama K

The novel calmodulin-binding site of the smooth muscle myosin light chain kinase

平滑肌肌球蛋白轻链激酶的新钙调蛋白结合位点

• 发表时间: 2010
• 作者: Nakamura;A.;Matsumoto;A.;Xie;C.;Yoshiyama;S.;Ishikawa;R.;Kohama;K.
• 通讯作者: K.

Nonkinase activity of MLCK in elongated filopodia formation and chemotaxis of vascular smooth muscle cells toward sphingosylphosphorylcholine.

• DOI: 10.1152/ajpheart.00965.2008
• 发表时间: 2009-02
• 期刊: American journal of physiology. Heart and circulatory physiology
• 作者: Hong Hui Wang;A. Nakamura;A. Matsumoto;S. Yoshiyama;Xiao-ran Qin;L. Ye;Ce Xie;Yue Zhang;Ying Gao;R. Ishikawa;K. Kohama

Modulation of the mechano-chemical properties of myosin V by drebrin-E

• DOI: 10.1016/j.bbrc.2010.08.120
• 发表时间: 2010-10-01
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Kubota, Hiroaki;Ishikawa, Ryoki;Ishiwata, Shin'ichi
• 通讯作者: Ishiwata, Shin'ichi

Utilization of myosin and actin bundles for the transport of molecular cargo

利用肌球蛋白和肌动蛋白束运输分子货物

• 发表时间: 2010
• 期刊: Small
• 影响因子: 13.3
• 作者: Takatsuki H;Rice KM;Asano S;Day BS;Hino M;Oiwa K;Ishikawa R;Hiratsuka Y;Uyeda TQ;Kohama K;Blough ER
• 通讯作者: Blough ER