Analysis of myosin sliding on wide variety of actin-rails.

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To examine whether or not brain-specific actin binding protein drebrin affects the activity of myosin-V, we observed the sliding of myosin-V on F-actin in the presence or absence of drebrin. We found that myosin-V slides on drebrin-decorated F-actin as fast as "naked" F-actin, whereas sliding distance and attachment time were smaller in the presence of drebrin than that in the absence of drebrin. Drebrin inhibits the binding of leading head to F-actin, but does not inhibit the release of ADP from trailing head. These results suggest that drebrin modulates the affinity of myosin-V to F-actin, thus inhibits the activity of myosin-V, and affects the motility of the central zone of nerve cell growth cones.