Fecal-based long DNA assay for colorectal cancer detection

基于粪便的长 DNA 检测用于结直肠癌检测

• 批准号: 21590626
• 负责人: SUEHIRO Yutaka
• 金额: $ 3.16万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590626/
• 关键词: 便DNA検査; 大腸がん; 大腸癌

Purpose : Although the fecal occult blood test is performed daily as a primary screening test, sensitivity is low, and false positive reactions may occur when hemorrhoids are present.Fecal DNA analysis appears to be a valid alternative method providing more advanced and reliable detection of CRC. Thus, we evaluated whether the DNA test could improve diagnostic performance for CRC detection.Methods : DNA was isolated from stools of patients with CRC and Twist1 methylation level was evaluated using pyrosequencing.Results : We found TWIST1 methylation occurred in feces with a sensitivity of 18% for detection of CRC.Conclusions : TWIS1 methylation assay may be useful for the detection of CRC.

目的：虽然粪便潜血试验是每天进行的主要筛查试验，敏感性低，假阳性反应时可能会出现痔疮present.Fecal DNA分析似乎是一个有效的替代方法，提供更先进和可靠的检测CRC。方法：从结直肠癌患者粪便中提取DNA，采用焦磷酸测序法检测Twist 1甲基化水平。结果：发现TWIST 1甲基化存在于粪便中，其检测结直肠癌的敏感性为18%。结论：TWIS 1甲基化检测对结直肠癌的诊断有一定的价值。

项目成果

大腸腫瘍の検出方法

如何检测结直肠肿瘤

• 发表时间: 2009

大腸がんgenomicsおよびepigenomicsの臨床検査への応用

结直肠癌基因组学和表观基因组学在临床检测中的应用

• 发表时间: 2011
• 作者: 渡邉幹夫;井上直哉、森田麻美、富澤理恵、赤水尚史、巽圭太、日高洋、岩谷良則;岡田季之;末広寛
• 通讯作者: 末広寛

大腸腫癌の検出方法

如何检测结直肠癌

• 发表时间: 2009

大腸癌の検出方法

如何检测结直肠癌

• 发表时间: 2018

TWIST1 Hypermethylation Is Observed Frequently in Colorectal Tumors and its Overexpression Is Associated with Unfavorable Outcomes in Patients with Colorectal Cancer

• DOI: 10.1002/gcc.20755
• 发表时间: 2010-05-01
• 期刊: GENES CHROMOSOMES & CANCER
• 影响因子: 3.7
• 作者: Okada, Toshiyuki;Suehiro, Yutaka;Hinoda, Yuji
• 通讯作者: Hinoda, Yuji