Analysis of the Crosstalk between Myocardium and Vasculature:Mechanism of Prevention of Hypertension by Intervention in Prehypertensive Stage
Analysis of the Crosstalk between Myocardium and Vasculature:Mechanism of Prevention of Hypertension by Intervention in Prehypertensive Stage
批准号:
21590943
负责人:
KAI Hisashi
金额:
$2.91万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
我们建立了一个选择性和全面的基因表达模式分析分离心肌和血管在心脏中使用激光显微切割(LMD)的方法。为探讨高血压前期药物干预对高血压发展的影响,对4-7周龄的易卒中型自发性高血压大鼠(SHR-SP)给予血管紧张素II受体阻滞剂(ARB)。早期接受ARB治疗的SHR-SP在高血压阶段的血压明显低于未接受ARB治疗的SHR-SP。ARB早期暴露的SHR-SP的脑卒中发生率和全因死亡发生率均显著低于未暴露的SHR-SP。比较ARB早期暴露与未暴露SHR-SP心肌面积和血管面积mRNA表达的差异。通过对原发性高血压大鼠行去窦-主动脉神经术,建立了高血压和大血压变异性(BPV)复合的大鼠模型。在血压正常的大鼠中,BPV增加仅引起有限的心脏肥大和纤维化。相反,大BPV加重了SHR的心脏肥大和纤维化,导致LV收缩功能障碍。结果表明,心肌内小动脉的慢性炎症引起了大BPV引起的心脏重构加重。分别研究了高血压和大BPV组合特异性诱导的心肌区和血管区基因表达谱的变化。
英文摘要
We established a selective and comprehensive gene expression pattern analysis separating the myocardium and vasculature in the heart using the laser microdissection(LMD) method. To investigate the effects of pharmacological intervention at the prehypertensive phase on the development of hypertension, an angiotensin II receptor blocker(ARB) was administered to stroke-prone spontaneously hypertensive rats(SHR-SP) of 4-7 weeks old. SHR-SP with early exposure to ARB showed significantly lower blood pressure at hypertensive stage than those without ARB treatment. Also, the incidence of stroke and the incidence of all cause death were significantly lower in SHR-SP with ARB early exposure than those without ARB. The mRNA expression patterns of the myocardium area and vascular area were being compared separately between SHR-SP with and without ARB early exposure. A rat model of a combination of hypertension and large blood pressure variability(BPV) was created by performing a sino-aortic denervation in spoitaneously hypertensive rats. In normotensive rats, increased BPV induced only limited cardiac hypertrophy and fibrosis. In contrast, large BPV exaggerated cardiac hypertrophy and fibrosis, leading to LV systolic dysfunction in SHR. It was reveled that chronic inflammation of the intramyocardial arterioles elicited the large BPV-induced aggravation of cardiac remodeling. The gene expression profile changes induced specifically by a combination of hypertension and large BPV were being investigated separately in the myocardium area and vascular area.
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Low diastolic blood pressure may not be an independent risk for cardiovascular death in revascularized coronary artery disease patients
低舒张压可能不是血运重建冠心病患者心血管死亡的独立风险
DOI:
--
发表时间:
2011
期刊:
J Hypertens
影响因子:
4.9
作者:
[Kai H, (他6名1番目)]
通讯作者:
(他6名1番目)
Inhibition of CaMKII-and ERK-mediated eNOS phosphorylation impairs endothelial function in renal failure rats : New effect of asymmetric dimethylarginine
抑制 CaMKII 和 ERK 介导的 eNOS 磷酸化会损害肾衰竭大鼠的内皮功能:不对称二甲基精氨酸的新作用
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Kajimoto H, Kai H, Aoki Y, Yasuoka S, Anegawa T, Aoki H, Imaizumi T]
通讯作者:
Imaizumi T
Eplerenone Prevented the Large Blood Pressure Variability-Induced Aggravation of Hypertensive Cardiac Remodeling and Left Ventricular Dysfunction in Spontaneously Hypertensive Rats
依普利农预防自发性高血压大鼠血压大变异引起的高血压心脏重塑和左心室功能障碍
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[安岡逸, 甲斐久史, 工藤博司, 高山成政, 梶本英美, 姉川敬裕, 今泉勉]
通讯作者:
今泉勉
Autologous transplantation of bone marrow mononuclear cells improved ischemic peripheral neuropathy in humans
自体骨髓单核细胞移植可改善人类缺血性周围神经病
DOI:
10.1016/j.jacc.2010.02.050
发表时间:
2010
期刊:
J Am Coll Cardiol
影响因子:
24
作者:
[Arima K, Kai H, Imaizumi T(他8人11番目)]
通讯作者:
Imaizumi T(他8人11番目)
Mesenchymal stem cell-based prostacyclin synthase gene therapy for pulmonary hypertension in rats
基于间充质干细胞的前列环素合酶基因治疗大鼠肺动脉高压
DOI:
--
发表时间:
2010
期刊:
Basic Res Cardiol
影响因子:
9.5
作者:
[Takemiya K, Kai H, (他4名2番目)]
通讯作者:
(他4名2番目)
共 30 条
Key Molecule of Aggravation of Hypertensive Organ Damage by Large Blood Pressure Variability
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批准号:24591104
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.41万
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财政年份:2012
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负责人:KAI Hisashi
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依托单位:
Gender differences in the mechanism of hypertensive organ damage in the heart
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批准号:19590839
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.91万
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财政年份:2007
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负责人:KAI Hisashi
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依托单位:
Roles of inflammation in hypertensive organ damages
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批准号:17590768
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.92万
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财政年份:2005
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负责人:KAI Hisashi
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依托单位:
EFFECTS OF EXAGGERATED BLOOD PRESSURE VARIABILITY ON CARDIAC REMODELING AND PERIVASCULAR INFLAMMATION
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批准号:15590780
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.24万
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财政年份:2003
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负责人:KAI Hisashi
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依托单位:
Study on Numerical Simulation of Flow around Body by MPS using Cluster System
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批准号:15560691
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.3万
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财政年份:2003
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负责人:KAI Hisashi
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依托单位:
A Study on Wake Geometry behind Marine Proellers
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批准号:13650966
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.92万
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财政年份:2001
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负责人:KAI Hisashi
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依托单位:
NEW TREATMENT FOR DIASTOLIC DYSFUNCTION BY PREVENTING CARDIAC FIBROSIS -GENE THERAPY USING A MUTANT TGF-β, RECEPTOR-
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批准号:13670767
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.24万
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财政年份:2001
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负责人:KAI Hisashi
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依托单位:
Prevention of Cardiac9 Remodeling and Diastolic Dysfunction by inhibiting Fibrotic Process.
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批准号:12670711
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.3万
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财政年份:2000
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负责人:KAI Hisashi
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依托单位:
海外基金