Activation of intestinal mucosal immunization via probiotic strain with the mechanism of Cd1d and NKT cell

益生菌通过Cd1d和NKT细胞机制激活肠粘膜免疫

• 批准号: 21591301
• 负责人: MATSUMOTO Tetsuya
• 金额: $ 2.91万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591301/
• 关键词: 腸管免疫; 腸内細菌; bacterial translocation; 免疫不全; CD1d; NKT細胞; 菌血症; プロバイオティクス; germ-free; 緑膿菌

We studied the host mechanisms involved in controlling intestinal colonization with commensal bacteria. Under specific pathogen. free or germ-free conditions, intragastric administration of Pseudomonas aeruginosa, E. coli, Staphylococcus aureus, or Lactobacillus gasseri resulted in increased colonization of the small intestine and bacterial translocation in mice lacking Cd1d, compared with wild type mice. In contrast, activation of Cd1d-restricted T cells(NKT cells) withα-galactosylceramide caused diminished intestinal colonization with the same bacterial strains. In vitro data suggest that NKT cells were shown to induce the release of lysozyme from intestinal crypts. These data support a role for Cd1d in regulating intestinal colonization through mechanisms that include the control of Paneth cell function.

我们研究了控制共生细菌肠道定植所涉及的宿主机​​制。在特定病原体下。在自由或无菌条件下，与野生型小鼠相比，在缺乏 Cd1d 的小鼠中，灌胃铜绿假单胞菌、大肠杆菌、金黄色葡萄球菌或加氏乳杆菌会导致小肠定植和细菌易位增加。相比之下，用 α-半乳糖神经酰胺激活 Cd1d 限制性 T 细胞（NKT 细胞）会导致相同细菌菌株的肠道定植减少。体外数据表明 NKT 细胞可诱导肠隐窝释放溶菌酶。这些数据支持 Cd1d 通过控制潘氏细胞功能等机制调节肠道定植的作用。

项目成果

Oral administration of heat-killed Lactobacillus plantarum strain b240 protects mice against Salmonella enterica serovar Typhimurium.Bioscience

口服热灭活植物乳杆菌 b240 菌株可保护小鼠免受鼠伤寒沙门氏菌的侵害。生物科学

• 发表时间: 2010
• 期刊: Biotechnology, and Biochemistry
• 作者: 祖母井庸之;ほか;Ishikawa H
• 通讯作者: Ishikawa H

Measuring antimicrobial susceptibility of Pseudomonas aeruginosa using Poloxamer 407 gel

使用泊洛沙姆 407 凝胶测量铜绿假单胞菌的抗菌敏感性

• DOI: 10.1007/s10156-010-0109-x
• 发表时间: 2011
• 期刊: J Infect Chemother
• 影响因子: 2.2
• 作者: Yamada H;Koike N;Ehara T;Matsumoto T
• 通讯作者: Matsumoto T

Current Topics of Infectious Diseases in Japan and Asia

日本和亚洲传染病的当前话题

• 发表时间: 2010
• 作者: Hirao S;Wada H;Goto H;et al.;Matsumoto T
• 通讯作者: Matsumoto T

多剤耐性菌をめぐる最近の話題

围绕多重耐药细菌的最新主题

• 发表时间: 2012
• 作者: Shosaku Nomura;Tomoki Ito;et al;松本哲哉
• 通讯作者: 松本哲哉

Involvement of endotoxin in the mortality of mice with gut-derived sepsis due to Methicillin Resistant Staphylococcus aureus

内毒素与耐甲氧西林金黄色葡萄球菌引起的肠源性败血症小鼠的死亡有关

• 发表时间: 2010
• 期刊: Microbiology & Immunology
• 影响因子: 2.6
• 作者: Kuwata;T.;Igarashi;T.;Matsushita;S.;Uramatsu M
• 通讯作者: Uramatsu M