权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The study for the relationship between the quantity of Ascaris antigen in the house dust and asthma symptoms of children living in the rural area of Bangladesh

孟加拉国农村地区室内灰尘中蛔虫抗原含量与儿童哮喘症状关系的研究

基本信息

批准号：
21591373
负责人：
IWATA Tsutomu
金额：
$ 3万
依托单位：
Tokyo Kasei University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

So-called hygiene hypothesis means that better hygienic environment introduce less allergic diseases. This hypothesis is still controversial especially in the developing country where the ascaris infection is mostly endemic. We had published a paper concerning the epidemiological data which showed the morbidity of wheezing is about 16% in Bangladeshi children. The morbidity of ascaris infestation is over 70% in the rural area in Bangladesh. Our data threw the issue against the hygiene hypothesis. We had also showed that high amount of anti-ascaris IgE antibody significantly correlated to the morbidity of wheezy episodes, diagnosed as the bronchial asthma. We hypothesized there might be ascaris antigen in the house-dust which worked as the inhalant allergen. We surveyed the same field in the rural Bangladesh as the one surveyed in 2001.ISAAC questionnaire was used. Sixty one children out of 142(43%) who were wheezy group in 2001 showed wheezing episode yet after 10 years and 79 out of 97(81%) who were not wheezy showed no wheezing episode. This difference was statistically significant by chi square test(p<0.0001). House dust was collected from the bedding of these two groups. Higher amount of ascaris antigen(5.25μg/g dust, 1.15～ 9.36, 95% CI) was detected in the house dust of the wheezy group than the non-wheezy group(2.54μg/g dust, 1.67～ 3.41, 95% CI), although statistically not significant. The newly developed method for detecting ascaris antigen has some limitation, because something was detected from the dust which was collected from Japanese housing. Our result suggested that there might be cross-reactive materials other than ascaris antigen in those house dust collected from two countries. Further research is needed.

所谓卫生假说是指较好的卫生环境较少引起过敏性疾病。这一假设仍然是有争议的，特别是在发展中国家，蛔虫感染主要是地方性的。我们发表了一篇关于流行病学数据的论文，该数据显示孟加拉国儿童喘息的发病率约为16%。孟加拉国农村地区蛔虫感染的发病率超过70%。我们的数据使这个问题与卫生假说相悖。高水平的抗蛔虫IgE抗体与喘息发作的发生率显著相关，诊断为支气管哮喘。我们推测室内灰尘中可能含有蛔虫抗原，作为吸入性过敏原。我们调查了孟加拉国农村的同一个领域，在2001年的调查。2001年喘息组142例中有61例（43%）10年后仍有喘息发作，97例无喘息组中有79例（81%）10年后无喘息发作。通过卡方检验，该差异具有统计学显著性（p<0.0001）。从这两组的床上收集房屋灰尘。喘息组屋尘中蛔虫抗原含量（5.25μg/g尘，1.15~ 9.36，95% CI）高于非喘息组（2.54μg/g尘，1.67~ 3.41，95% CI），但差异无统计学意义。新开发的检测蛔虫抗原的方法存在一定的局限性，因为从日本住宅收集的灰尘中检测到了一些东西。结果提示，两国屋尘中除蛔虫抗原外，还可能存在交叉反应物质。需要进一步研究。