New melanoma model using transgenic medaka fish

本研究旨在构建稳定的荷瘤medaka细胞系，并将其应用于药物筛选。我们拟在水箱中建立不需要解剖就能发现肿瘤的黑色素瘤模型，进行化学筛选。用Tg-hsp: cre杂交(Tg)-tyr: HRAS^<G12V> medaka与Tg-hsp: cre medaka，通过热处理获得medaka系诱导的人HRAS^<G12V>。在受精后6个月，所有的双Tg medaka都有黑色素细胞增生或黑色素瘤。索拉非尼对双Tg medaka肿瘤的初步治疗显示出明显的肿瘤生长抑制和延长寿命。

The purpose of this study is to construct the stable tumor bearing medaka line and to apply the line to drug screening. We intended to establish the melanoma model which can be detected tumors without autopsy for chemical screening in water tank. With cross transgenic(Tg)-tyr : HRAS^<G12V> medaka with Tg-hsp : cre medaka, we could obtain medaka line induced human HRAS^<G12V> by heat treatment. All of the double Tg medaka had melanophore hyperplasia or melanoma at six month post fertilization. Preliminary administration of sorafenib to this double Tg medaka having tumor showed significant inhibition of tumor growth and elongation of longevity.