New combinatorial approach to peritoneal dissemination of scirrhous gastric carcinoma based REIC/Dkk-3 gene therapy

基于 REIC/Dkk-3 基因治疗的硬质胃癌腹膜播散新组合方法

• 批准号: 21591699
• 负责人: KATAOKA Ken
• 金额: $ 2.66万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591699/
• 关键词: REIC/Dkk-3; Gene Therapy; Scirrhous Gastric Carcinoma; Peritoneal Dissemination; REIC; Dkk-3

A single intraperitoneal injection of REIC/Dkk-3 adenovirus suppressed tumor dissemination and disease progression of scirrhous gastric carcinoma(SGC) in the mouse model. Immunomodulation by Ad-REIC led to recruitment of natural killer cells inside tumor nodules. We conclude that REIC/Dkk-3 gene therapy may be a potential tool in combinatorial approaches to achieve curative effects in SGC. We also demonstrated that PINK1, a key molecule for the mitochondrial protection system, was a new target molecule to sensitize resistant cancer cells to REIC/Dkk-3 gene therapy.

在小鼠模型中，单次腹腔注射REIC/Dkk-3腺病毒抑制了硬化性胃癌（SGC）的肿瘤扩散和疾病进展。通过Ad-REIC的免疫调节导致肿瘤结节内自然杀伤细胞的募集。我们的结论是，REIC/Dkk-3基因治疗可能是一个潜在的工具，在组合的方法，以达到治疗效果的SGC。我们还证明了PINK 1，线粒体保护系统的关键分子，是一种新的靶分子，使耐药癌细胞对REIC/Dkk-3基因治疗敏感。

项目成果

Application of a thermo-reversible gelation polymer, mebiol gel, for stem cell culture and regenerative medicine.

热可逆聚合物Mebiol凝胶的应用用于干细胞培养和再生医学。

• DOI: 10.46582/jsrm.0601003
• 发表时间: 2010
• 期刊: Journal of stem cells & regenerative medicine
• 影响因子: 2.7
• 作者: Kataoka K;Huh N
• 通讯作者: Huh N

Reduced expression of REIC/Dkk-3, a putative tumor suppressor gene, in hyperproliferativeepidermis

REIC/Dkk-3（一种假定的抑癌基因）在过度增殖性表皮中的表达减少

• 发表时间: 2010
• 作者: Du G;Kataoka K;Sakaguchi M;Makino T;Shimizu T;Huh NH
• 通讯作者: Huh NH

Impact of Bcl-xL deficiency in mouse keratinocytes on susceptibility to both UVB-and chemically-induced skin carcinogenesis

小鼠角质形成细胞中 Bcl-xL 缺陷对 UVB 和化学诱导的皮肤癌易感性的影响

• 发表时间: 2009
• 作者: Kim;DJ;Kataoka K;Sano S;Kiguchi K;DiGiovanni J
• 通讯作者: DiGiovanni J

REIC/Dkk-3 stable transfection reduces the malignant phenotype of mouse prostate cancer RM9 cells

• DOI: 10.3892/ijmm_00000293
• 发表时间: 2009-12-01
• 期刊: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
• 影响因子: 5.4
• 作者: Chen, Jie;Watanabe, Masami;Kumon, Hiromi
• 通讯作者: Kumon, Hiromi

"PINK1 knockdown increases the apoptosis induced by adenovirus-mediated REIC/Dkk-3 overexpression on bladder cancer cells "

“PINK1 敲低会增加腺病毒介导的 REIC/Dkk-3 过表达膀胱癌细胞诱导的细胞凋亡”

• 作者: "YU JIN;HITOSHI MURATA;KEN KATAOKA;MASAKIYO SAKAGUCHI;NAM-HO HUH"
• 通讯作者: NAM-HO HUH"