The development of new treatment for improvement of microcirculation in cirrhotic liver

改善肝硬化微循环新疗法的开发

• 批准号: 21591755
• 负责人: AKAHOSHI Tomohiko
• 金额: $ 3万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591755/
• 关键词: 肝硬変; 肝微小循環; Rho kinase, eNOS; ET-1; Nitric oxide (NO; ナノカプセル; rho-kinase; VEGF; 脾臓; 門脈圧亢進症; Rho kinase阻害剤; Shear response factor

Study 1. RhoA and Rho kinase expressions were significantly over expressed in cirrhotic liver accompanied with decrease in eNOS activity. Splenectomy significantly reduce portal vein pressure and improved disturbance of microcirculation in the cirrotic liver. In the liver of cirrhotic rat, Splenectomy also decreased serum ET-1 levels. The ET-1 level was strongly related RhoA activation, and decrease of eNOS phosphorylation. ET-1 derived from the spleen increase intrahepatic resistance by down-regulating Rho signaling in liver cirrhosis.Study 2. Splenectomy induced thrombocytosis, and increased serotonin content in cirrhotic liver. Stimulation of liver regeneration was indicated by the following parameters : hepatocyte ratio to the entire liver area, Ki67-positive hepatocyte count, and expression of phosphorylated extracellularsignal-regulated kinases. This enhancement of liver regenerationwas negated by ketanserin. Our results showed that splenectomy promoted liver regeneration by increasing serotonin content in liver even under cirrhotic conditions.

研究1。肝硬化组织中RhoA和Rho激酶的表达明显过表达，eNOS活性降低。脾切除术可显著降低门静脉压力，改善肝硬化肝脏微循环障碍。在肝硬化大鼠肝脏中，脾切除术也能降低血清ET-1水平。ET-1水平与RhoA激活和eNOS磷酸化降低密切相关。脾源ET-1通过下调肝硬化Rho信号增加肝内抵抗。研究2。脾切除术导致肝硬化患者血小板增多，血清素含量升高。肝再生的刺激通过以下参数显示：肝细胞占整个肝面积的比例，ki67阳性肝细胞计数和磷酸化的细胞外信号调节激酶的表达。这种肝脏再生的增强作用被酮色林所抵消。我们的研究结果表明，脾切除术通过增加肝脏中的血清素含量来促进肝脏再生，即使在肝硬化的情况下。

项目成果

Risk factors for portal venous thrombosis after splenectomy in patients with cirrhosis and portal hypertension

• DOI: 10.1016/s0739-5930(10)79588-x
• 发表时间: 2010
• 期刊: Yearbook of Gastroenterology
• 作者: S. Bollipo;V. Ambikaipaker;N. Talley

当科における食道胃静脈瘤における治療の変遷と成績

我科食管胃静脉曲张治疗的变化及结果

• 发表时间: 2011
• 作者: 赤星朋比古;富川盛雅;橋爪 誠
• 通讯作者: 橋爪 誠

Impact of Antithrombin III Concentrates on Portal Vein Thrombosis After Splenectomy in Patients With Liver Cirrhosis and Hypersplenism

• DOI: 10.1097/sla.0b013e3181bdf8ad
• 发表时间: 2010-01-01
• 期刊: ANNALS OF SURGERY
• 影响因子: 9
• 作者: Kawanaka, Hirofumi;Akahoshi, Tomohiko;Maehara, Yoshihiko
• 通讯作者: Maehara, Yoshihiko

腹腔鏡下脾臓摘出術のlatest modern technique

腹腔镜脾切除术的现代最新技术

• 发表时间: 2011
• 作者: 赤星朋比古;橋爪誠;赤星朋比古;赤星朋比古;赤星朋比古
• 通讯作者: 赤星朋比古

難治性消化管静脈瘤に対する外科治療の意義

难治性胃肠静脉曲张手术治疗的意义

• 发表时间: 2011
• 作者: 赤星朋比古;他
• 通讯作者: 他