The development of new treatment for improvement of microcirculation in cirrhotic liver

研究1。肝硬化组织中RhoA和Rho激酶的表达明显过表达，eNOS活性降低。脾切除术可显著降低门静脉压力，改善肝硬化肝脏微循环障碍。在肝硬化大鼠肝脏中，脾切除术也能降低血清ET-1水平。ET-1水平与RhoA激活和eNOS磷酸化降低密切相关。脾源ET-1通过下调肝硬化Rho信号增加肝内抵抗。研究2。脾切除术导致肝硬化患者血小板增多，血清素含量升高。肝再生的刺激通过以下参数显示：肝细胞占整个肝面积的比例，ki67阳性肝细胞计数和磷酸化的细胞外信号调节激酶的表达。这种肝脏再生的增强作用被酮色林所抵消。我们的研究结果表明，脾切除术通过增加肝脏中的血清素含量来促进肝脏再生，即使在肝硬化的情况下。

Study 1. RhoA and Rho kinase expressions were significantly over expressed in cirrhotic liver accompanied with decrease in eNOS activity. Splenectomy significantly reduce portal vein pressure and improved disturbance of microcirculation in the cirrotic liver. In the liver of cirrhotic rat, Splenectomy also decreased serum ET-1 levels. The ET-1 level was strongly related RhoA activation, and decrease of eNOS phosphorylation. ET-1 derived from the spleen increase intrahepatic resistance by down-regulating Rho signaling in liver cirrhosis.Study 2. Splenectomy induced thrombocytosis, and increased serotonin content in cirrhotic liver. Stimulation of liver regeneration was indicated by the following parameters : hepatocyte ratio to the entire liver area, Ki67-positive hepatocyte count, and expression of phosphorylated extracellularsignal-regulated kinases. This enhancement of liver regenerationwas negated by ketanserin. Our results showed that splenectomy promoted liver regeneration by increasing serotonin content in liver even under cirrhotic conditions.