Functional analyses of mammalian round spermatid for fertilization

哺乳动物圆形精子细胞受精功能分析

• 批准号: 21592086
• 负责人: HAYASAKA Shinichi
• 金额: $ 2.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592086/
• 关键词: 円形精子細胞; 精子中心体; 補助活性化法; 受精障害; 生殖補助技術; 受精能; 発生能; 不妊症

The goal of the fertilization assumes he fusion of the sex genome in the egg cytoplasm. We examined fertilization establishment ability of the round spermatid of the mammal that was assumed to be extremely low, using human and a laboratory animal (a mouse, rabbit). Sperm function to accomplish a major event of the fertilization after the sperm entry, oocyte activation ability and sperm centrosomal function were found to be decreased. Furthermore, as one of the methods to supplement ability for oocyte activation that decreased with round spermatid, we developed the co-culture system with hormone addition and Sertoli cells of the mouse round spermatid.

受精的目的是假设性基因组在卵细胞质中融合。我们研究了受精建立能力的圆形精子细胞的哺乳动物，被认为是非常低的，使用人类和实验室动物（小鼠，兔）。精子进入后，精子完成受精重大事件的功能、卵母细胞激活能力和精子中心体功能均下降。此外，作为补充圆形精子细胞降低的卵母细胞激活能力的方法之一，我们开发了添加激素的小鼠圆形精子细胞与Sertoli细胞的共培养系统。

项目成果

A novel culture system for mouse spermatid maturation which produces elongating spermatids capable of inducing calcium oscillation during fertilization and embryonic development

• DOI: 10.1007/s10815-010-9442-3
• 发表时间: 2010-09-01
• 期刊: JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
• 影响因子: 3.1
• 作者: Hasegawa, Hisataka;Terada, Yukihiro;Sato, Kahei
• 通讯作者: Sato, Kahei

Aberrant behavior of mouse embryo development after blastomere biopsy as observed through time-lapse cinematography

通过延时摄影观察卵裂球活检后小鼠胚胎发育的异常行为

• 发表时间: 2010
• 期刊: Fertil Steril
• 影响因子: 6.7
• 作者: UgajinT;Terada Y;Hasegawa H;Velayo CL;Nabeshima H;Yaegashi N
• 通讯作者: Yaegashi N

Laparoscopic management of recurrent rupture of an adnexal mass in the second trimester of pregnancy

妊娠中期附件包块复发性破裂的腹腔镜治疗

• 发表时间: 2010
• 期刊: A case report Asian Journal of Endoscopic Surgery
• 作者: Hayasaka S;Murakami T,Nabeshima H;Terada Y;Yaegashi N
• 通讯作者: Yaegashi N

Essential roles of the sperm centrosome in human fertilization: developing the therapy for fertilization failure due to sperm centrosomal dysfunction.

• DOI: 10.1620/tjem.220.247
• 发表时间: 2010-04
• 期刊: The Tohoku journal of experimental medicine
• 作者: Y. Terada;G. Schatten;H. Hasegawa;N. Yaegashi

Laparoscopic removal of the rudimental uterus followed by colpopoiesis in a Mayer-Rokitansky-Kuster-Hauser syndrome patient with a functional endometrium and peritoneal endometriosis

对患有功能性子宫内膜和腹膜子宫内膜异位症的 Mayer-Rokitansky-Kuster-Hauser 综合征患者进行腹腔镜切除残子宫并进行阴道生成术

• 发表时间: 2010
• 期刊: J Gynecol Surg
• 影响因子: 0.3
• 作者: Terada Y;Ugajin T;Arai-Kikuchi M;Hayasaka S;Nabeshima H;Yaegashi N
• 通讯作者: Yaegashi N