Interaction between platelet and trophoblast-Toward the elucidation of pathophysiology of PIH and IUGR-

血小板和滋养层之间的相互作用-阐明 PIH 和 IUGR 的病理生理学-

• 批准号: 21592095
• 负责人: SATO Yukiyasu
• 金额: $ 2.91万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592095/
• 关键词: 絨毛外トロホブラスト; 血小板; 浸潤; 血管リモデリング; 妊娠高血圧症候群; 子宮内胎児発育遅延

Using extravillous trophoblast(EVT) isolated from chorionic villous explant cultures, we successfully reproduced EVT transformation towards endovascular phenotype by co-culturing with platelets. Microarray analysis revealed that platelet co-culture up-regulates several molecules, which we regarded as candidates for endovascular EVT markers. Immunohistochemistry of human placental tissues showed that one of such candidates, MCAM(CD146), is highly expressed on endovascular trophoblast. Now, we are planning to perform functional analysis for MCAM using isolated EVT.In addition, we demonstrated that sphingosine-1-phosphate, one of platelet-derived factors, promotes invasion of the isolated EVT. This supports our hypothesis that interaction between maternal platelets and EVT plays an important role in maternal vascular remodeling.

使用绒毛外滋养层细胞（EVT）分离的绒毛膜绒毛外植体培养物，我们成功地再现EVT转化为血管内表型与血小板共培养。微阵列分析显示，血小板共培养上调了几种分子，我们认为这些分子是血管内EVT标记物的候选者。人胎盘组织的免疫组织化学显示，这样的候选人之一，MCAM（CD 146），是血管内滋养层的高表达。目前，我们正计划利用孤立的EVT对MCAM进行功能分析，另外，我们证明了血小板衍生因子之一的鞘氨醇-1-磷酸促进孤立EVT的侵袭。这支持了我们的假设，母体血小板和EVT之间的相互作用在母体血管重塑中起着重要作用。

项目成果

Immune System Cooperatively Supports Endocrine System-Primed Embryo Implantation

• DOI: 10.1274/jmor.26.122
• 发表时间: 2009-12
• 作者: H. Fujiwara;A. Ideta;Y. Araki;Y. Takao;Yukiyasu Sato;N. Tsunoda;Y. Aoyagi;I. Konishi

Prenatal findings incongenital leukemia : a case report

产前检查结果非先天性白血病：病例报告

• DOI: 10.1159/000323582
• 发表时间: 2011
• 期刊: Fetal Diagn Ther
• 影响因子: 2.2
• 作者: Sato Y;Izumi Y;Minegishi K;Komada M;Yamada S;Kakui K;Tatsumi K;Mikami Y;Fujiwara H and Konishi I
• 通讯作者: Fujiwara H and Konishi I

Intrauterine administration of autologous peripheral blood mononuclear cells increases clinical pregnancy rates in frozen/thawed embryo transfer cycles of patients with repeated implantation failure

• DOI: 10.1016/j.jri.2011.07.001
• 发表时间: 2011-12-01
• 期刊: JOURNAL OF REPRODUCTIVE IMMUNOLOGY
• 影响因子: 3.4
• 作者: Okitsu, Osamu;Kiyokawa, Machiko;Fujiwara, Hiroshi
• 通讯作者: Fujiwara, Hiroshi

Prenatal diagnosis ofanterior sacral meningocele

骶前部脑膜膨出的产前诊断

• DOI: 10.1002/uog.8852
• 发表时间: 2011
• 期刊: Ultrasound Obstet Gynecol
• 作者: Sumi A;Sato Y;Kakui K;Tatsumi K;Fujiwara H and Konishi I.
• 通讯作者: Fujiwara H and Konishi I.

Ephrin A1 induces intercellular dissociation in Ishikawa cells: possible implication of the Eph-ephrin A system in human embryo implantation

• DOI: 10.1093/humrep/deq340
• 发表时间: 2011-02-01
• 期刊: HUMAN REPRODUCTION
• 影响因子: 6.1
• 作者: Fujii, Haruko;Fujiwara, Hiroshi;Konishi, Ikuo
• 通讯作者: Konishi, Ikuo