Interaction between platelet and trophoblast-Toward the elucidation of pathophysiology of PIH and IUGR-

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Using extravillous trophoblast(EVT) isolated from chorionic villous explant cultures, we successfully reproduced EVT transformation towards endovascular phenotype by co-culturing with platelets. Microarray analysis revealed that platelet co-culture up-regulates several molecules, which we regarded as candidates for endovascular EVT markers. Immunohistochemistry of human placental tissues showed that one of such candidates, MCAM(CD146), is highly expressed on endovascular trophoblast. Now, we are planning to perform functional analysis for MCAM using isolated EVT.In addition, we demonstrated that sphingosine-1-phosphate, one of platelet-derived factors, promotes invasion of the isolated EVT. This supports our hypothesis that interaction between maternal platelets and EVT plays an important role in maternal vascular remodeling.