Experimental study of vascularized flap preparation in a tissue engineering chamber

组织工程室内血管化皮瓣制备的实验研究

• 批准号: 21592289
• 负责人: TANAKA Yoshio
• 金额: $ 3万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592289/
• 关键词: 多血小板血漿; FGF-2; 細胞増殖因子; 細胞外マトリックス; 再生組織; 多血漿板血漿; FGF; 再生医療; 血管付き軟組織; tissue engineering chamber; 骨髄液由来多血小板血漿; 線維芽細胞増殖因子

We evaluated a method to regenerate soft tissue flap in a tissue engineering chamber(TEC) as a site of regeneration by concomitantly using an AV bundle, artificial dermis, FGF-2, and platelet-rich plasma(PRP). The samples were divided into control, FGF-2, non-activated PRP, activated PRP, non-activated PRP+ FGF-2, and activated PRP+ FGF-2 groups, and the amount of regenerated tissue, degree of maturation of organization were evaluated. Results : Non-activated PRP induced more satisfactory regeneration of vascularized soft tissue than Activated PRP. Regenerated tissue with the highest degree of maturation was obtained in the non-activated PRP+ FGF-2group. The results indicate that the simultaneous use of non-activated PRP and FGF-2 is advantageous for the regeneration of vascularized soft tissue in a TEC.

我们评估了一种在组织工程室 (TEC) 中同时使用 AV 束、人造真皮、FGF-2 和富血小板血浆 (PRP) 作为再生部位的软组织瓣再生方法。将样品分为对照组、FGF-2组、非活化PRP组、活化PRP组、非活化PRP+FGF-2组、活化PRP+FGF-2组，评价再生组织量、组织成熟程度。结果：非活化 PRP 比活化 PRP 更能诱导血管软组织再生。在未激活的PRP+FGF-2组中获得了成熟程度最高的再生组织。结果表明，同时使用非活化 PRP 和 FGF-2 有利于 TEC 中血管化软组织的再生。