The regulation of cell proliferation and adhesion by chloride channel related factor CLCA.

氯通道相关因子 CLCA 对细胞增殖和粘附的调节。

• 批准号: 21592407
• 负责人: OKAMURA Kazuhiko
• 金额: $ 2.5万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592407/
• 关键词: イオンチャネル; CLCA; 細胞増殖; 分化; 癌の浸潤; クロライドチャネル; 発生・分化; 細胞接着; siRNA抑制; 再構築モデル; p63

A rat CLCA(originally named chloride channels, calcium-activated) homologue fCLCA has a splicing isoform trCLCA. Owing to the weak association of trCLCA to the other CLCA isoforms of other species, the morphological and functional significance of trCLCA has not been established. We have carried out the tissue localization of trCLCA in rat, with support of grants-in-aid for Scientific Research(C) 18592025.Subsequently, functional analyses of trCLCA was undertaken in this project, using in vitro system of reconstruction model of stratified squamous epithelium and several kinds of cultured cells. One of important result is : inhibition of trCLCA expression by siRNA bring out the change of cytokeratin profile, followed by negative regulation of cellular adhesion and proliferation.

大鼠CLCA（最初称为氯化物通道，钙激活）同源物fclCA具有剪接同工型TRCLCA。由于TRCLCA与其他物种的其他CLCA同工型的关联较弱，因此尚未建立TRCLCA的形态和功能意义。我们已经在大鼠中进行了TRCLA的组织定位，并支持了科学研究的赠款（C）185920255。随后，在该项目中，使用了分层鳞状上皮的重建模型的体外体系进行了TRCLCA的功能分析。重要的结果之一是：通过siRNA抑制TRCLCA表达，带来了细胞角蛋白谱的变化，然后是细胞粘附和增殖的阴性调节。

项目成果

Transformation from fibroblast to myofibroblast is regulated by TGFβ and mechanical tension in a rat skin reconstruction model

大鼠皮肤重建模型中成纤维细胞向肌成纤维细胞的转化受 TGFβ 和机械张力的调节

• 发表时间: 2012
• 作者: Hata S;Okamura K;Ishikawa H;Yamazaki J
• 通讯作者: Yamazaki J

Blockade of TRPM8 activity reduces the invasion potential of oral squamous carcinoma cell lines

• DOI: 10.3892/ijo.2012.1340
• 发表时间: 2012-05-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Okamoto, Yoshihiko;Ohkubo, Tsuyako;Yamazaki, Jun
• 通讯作者: Yamazaki, Jun

Expression of caspase-14 in stratified and cornified epithelium in a ratoral mucosal reconstruction model

口腔粘膜重建模型中复层和角化上皮中 caspase-14 的表达

• 发表时间: 2012
• 作者: Murakami H;Okamura K;Sakagami R;Yamazaki J
• 通讯作者: Yamazaki J

CLCA isoforms and structural proteins differentially expressed in a reconstuction model derived from rat skin

大鼠皮肤重建模型中 CLCA 亚型和结构蛋白的差异表达

• 发表时间: 2012
• 作者: Yamazaki J;Okamura K;Murakami H;Hata S;et al
• 通讯作者: et al