The regulation of cell proliferation and adhesion by chloride channel related factor CLCA.

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A rat CLCA(originally named chloride channels, calcium-activated) homologue fCLCA has a splicing isoform trCLCA. Owing to the weak association of trCLCA to the other CLCA isoforms of other species, the morphological and functional significance of trCLCA has not been established. We have carried out the tissue localization of trCLCA in rat, with support of grants-in-aid for Scientific Research(C) 18592025.Subsequently, functional analyses of trCLCA was undertaken in this project, using in vitro system of reconstruction model of stratified squamous epithelium and several kinds of cultured cells. One of important result is : inhibition of trCLCA expression by siRNA bring out the change of cytokeratin profile, followed by negative regulation of cellular adhesion and proliferation.