Mesenchymal stem cells-photocurable gelatin-based gelation material for application to periodontal regeneration

间充质干细胞-光固化明胶基凝胶材料在牙周再生中的应用

• 批准号: 21592501
• 负责人: NAKAGAWA Taneaki
• 金额: $ 2.75万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592501/
• 关键词: 再生歯学; 間葉系幹細胞; 歯周組織再生; 足場材料; 光硬化型ゼラチン; 細胞分化誘導; 組織再生; 足場; 幹細胞; 光硬化性ゼラチン; 動物実験

We confirmed that prospective isolatedMSCs showed a 120, 000-fold higher colony forming frequency thanthe conventional MSCs. Furthermore, Our results suggested that MSCs in adultbone marrow have at least two developmental origins, one of which is the neural crest. Furthermore, prospectively enrichedMSCs are a promising candidate for the efficient generation of high-quality iPS cells. This photocurable solution also has adequate viscosity at room temperature before curing, and it manipulation is enhanced. It is assumed that forms suitable for various bone defects will thus be easily rendered, and it is also assumed that the shape can be retained with the mechanical strength required by photocurable.

我们证实，预期分离的 MSCs 的集落形成频率比传统 MSCs 高 120, 000 倍。此外，我们的结果表明成人骨髓中的间充质干细胞至少有两个发育起源，其中之一是神经嵴。此外，前瞻性富集的 MSC 是有效生成高质量 iPS 细胞的有希望的候选者。该光固化溶液在固化前在室温下也具有足够的粘度，并且其操作性得到增强。据推测，适合各种骨缺损的形状将因此容易地呈现，并且还假设可以以光固化所需的机械强度保持形状。

项目成果

Mesp1+ early paraxial mesodermal cells supply initial bone marrow mesenchymal stem cells capable of differentiating into neural crest lineage cells.

Mesp1 早期近轴中胚层细胞提供能够分化为神经嵴谱系细胞的初始骨髓间充质干细胞。

• 发表时间: 2011
• 期刊: Inflammation and Regeneration 31(1)
• 作者: Niibe K;Morikawa S;Mabuchi Y;Araki D;Nakagawa T;Okano H;Matsuzaki Y
• 通讯作者: Matsuzaki Y

Inhibition of Abcg2 transporter on primitive hematopoietic stem cells by All-trans retinoic acid increases sensitivity to anthracycline

• DOI: 10.2492/inflammregen.30.55
• 发表时间: 2010-01
• 期刊: Inflammation and Regeneration
• 影响因子: 8.1
• 作者: Lawrence Lein;Y. Nagai;Y. Mabuchi;Sadafumi Suzuki;S. Morikawa;Y. Matsuzaki

Flow cytometric isolation and clonal identification of mesenchymal stem cells in human bone marrow

人骨髓间充质干细胞的流式细胞术分离与克隆鉴定

• 发表时间: 2009
• 作者: Mabuchi;Yo(発表者);Morikawa;Satoru;Suzuki;Sadafumi;Lein;Lawrence;Niibe;Kunimichi;Nagai;Yasuo;Sunabori;Takehiko;Okano;Hideyuki;Matsuzaki;Yumi;Yo Mabuchi
• 通讯作者: Yo Mabuchi

Anti-IL-6-receptor antibody promotes repair of spinal cord injury by inducing microglia-dominant inflammation

• DOI: 10.1016/j.expneurol.2010.04.020
• 发表时间: 2010-08-01
• 期刊: EXPERIMENTAL NEUROLOGY
• 影响因子: 5.3
• 作者: Mukaino, Masahiko;Nakamura, Masaya;Okano, Hideyuki
• 通讯作者: Okano, Hideyuki

ヒト間葉系幹細胞の予期的分離とClonal解析

人间充质干细胞的预期分离和克隆分析

• 发表时间: 2010
• 作者: 森川暁、新部邦透;他
• 通讯作者: 他