The analysis of the immune response to the virulence factors of the periodontopathic bacteria
牙周病菌毒力因子的免疫应答分析
基本信息
- 批准号:12672040
- 负责人:
- 金额:$ 2.18万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We initiated a study to determine whether RgpA and Kgp have opsonic target sites and whether these sites are available and accessible on intact P.gingivalis cells. Rabbits were immunized and IgG fractions were isolated from preimmune and immune sera. Functional characteristics of the antibodies were assessed by determining antibody titers by ELISA, generating Western blots, and measuring of the antibody enhancement of P.gingivalis opsonization, phagocytosis and killing by polymorphonuclear leukocytes of intact cells of P.gingivalis representative of the four serotypes. Both RgpA and Kgp induced high titers of IgG antibody. Anti-RgpA and anti-Kgp antibody bound to both RgpA and Kgp demonstrating a large proportion of shared antigenic epitopes. The two antibodies bound equally well to all four P.gingivalis serotypes compared to preimmune IgG. The immunoblot patterns of binding of the two antibodies to RgpA and Kgp and to sonlcates of the four P.gingivalis serotypes were virtually identical. Both proteins induced antibodies that significantly enhanced opsonization as assessed by chemiluminescence. Both antibodies significantly enhanced PMN-mediated bacterial killing of the four P.gingivalis serotypes. These data indicate these two proteins appear to be potential candidate antigens for an anti-P.gingivalis vaccine. We have similar data on RgpB, which possesses only a catalytic domain. The data indicate that RgpB also appear to be potential candidate antigen for anti-P.gingivalis vaccine
我们启动了一项研究,以确定RgpA和Kgp是否具有调理剂靶位点,以及这些位点在完整的牙龈卟啉单胞菌细胞上是否可用和可接近。免疫家兔,并从免疫前和免疫血清中分离IgG组分。通过ELISA测定抗体滴度,产生蛋白质印迹,并测量抗体对牙龈卟啉单胞菌调理作用的增强、吞噬作用以及代表四种血清型的牙龈卟啉单胞菌完整细胞的多形核白细胞的杀伤作用,来评估抗体的功能特征。RgpA和Kgp均诱导高滴度的IgG抗体。抗RgpA和抗Kgp抗体结合RgpA和Kgp两者,证明大比例的共享抗原表位。与免疫前IgG相比,这两种抗体与所有四种牙龈卟啉单胞菌血清型结合得同样好。两种抗体与RgpA和Kgp以及与四种牙龈卟啉单胞菌血清型的超声产物结合的免疫印迹模式几乎相同。这两种蛋白质诱导的抗体,显着增强调理作用,通过化学发光评估。两种抗体均显著增强PMN介导的对四种牙龈卟啉单胞菌血清型的细菌杀伤。这些数据表明这两种蛋白质似乎是抗牙龈卟啉单胞菌疫苗的潜在候选抗原。我们在RgpB上有类似的数据,它只具有催化结构域。提示RgpB也是抗牙龈卟啉单胞菌疫苗的潜在候选抗原
项目成果
期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Okuda, K. et al.: "Ecological and immunopathological implication of oral bacteria in Helicobacter pylori infected disease"J. Periodontol.. 74・1. 123-128 (2003)
Okuda, K. 等:“幽门螺杆菌感染性疾病中口腔细菌的生态学和免疫病理学意义”J. periodontol.. 74・1 (2003)。
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- 影响因子:0
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Sumida, S. et al.: "Transmission of periodontal disease associated bacteria from teeth to osseointegrated implant regions"Int J Oral Maxillofac Implants. 17・5. 696-702 (2002)
Sumida, S. 等人:“牙周病相关细菌从牙齿到骨整合种植体区域的传播”Int J Oral Maxillofac Implants 17·5 (2002)。
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稲垣 覚: "Porphyromonas gingivalis Arg-gingipainに対する歯周炎患者の免疫応答の解析"日歯周誌. 43. 240-250 (2001)
Satoru Inagaki:“牙周炎患者对牙龈卟啉单胞菌 Arg-gingipain 的免疫反应分析”《牙周病学杂志》43. 240-250 (2001)。
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- 影响因子:0
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Nakagawa,T., Sims,T., Fan,Q., Potempa,J., Travis,J., Houston,L., Page,R.C.: "Functional characteristics of antibodies induced by Arg-gingipain (HrgpA) and Lys-gingipain (Kgp) from Porphyromonas gingivalis"J Periodont Res. 16. 202-211 (2001)
Nakakawa,T.、Sims,T.、Fan,Q.、Potempa,J.、Travis,J.、Houston,L.、Page,R.C.:“Arg-gingipain (HrgpA) 和 Lys- 诱导的抗体的功能特征
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Adachi, M. et al.: "Effect of professional oral care on elderly living in nursing homes"Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology. 94・2. 191-195 (2002)
Adachi, M. 等:“专业口腔护理对疗养院老年人的影响”口腔外科口腔医学口腔病理学口腔放射学和牙髓病学 191-195(2002)。
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NAKAGAWA Taneaki其他文献
Oral Symptom Contributes to Diagnosis of Intestinal Behçet’s Disease:A Case Report
口腔症状有助于诊断肠道白塞氏病:病例报告
- DOI:
10.6014/jjsom.25.25 - 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
KOIKE Masato;IKEURA Kazuhiro;FUJITA Kohei;SHIMIZU Hiroyuki;KATO Shin;MIYASHITA Hidetaka;NAKAGAWA Taneaki;TSUNODA Kazuyuki - 通讯作者:
TSUNODA Kazuyuki
NAKAGAWA Taneaki的其他文献
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{{ truncateString('NAKAGAWA Taneaki', 18)}}的其他基金
Practical periodontal tissue regeneration therapy using highly purified dental pulp MSCs, induced neural crest cells, and induced odontogenic mesenchymal cells
使用高度纯化的牙髓间充质干细胞、诱导神经嵴细胞和诱导牙源性间充质细胞进行实用的牙周组织再生治疗
- 批准号:
18K09606 - 财政年份:2018
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Elucidation of chronic periodontitis tissue destruction and tissue remodeling mechanism as seen from the periodontal tissue stem cells
从牙周组织干细胞阐明慢性牙周炎组织破坏和组织重塑机制
- 批准号:
24592965 - 财政年份:2012
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mesenchymal stem cells-photocurable gelatin-based gelation material for application to periodontal regeneration
间充质干细胞-光固化明胶基凝胶材料在牙周再生中的应用
- 批准号:
21592501 - 财政年份:2009
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Effect of antimicrobial agent to artificial biofilm formed by periodontopathic bacteria
抗菌剂对牙周病菌人工生物膜的影响
- 批准号:
17592164 - 财政年份:2005
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
The Role of Gingipains in the Degradation of Dentogingival Attachment Proteins
牙龈蛋白酶在牙龈附着蛋白降解中的作用
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323933 - 财政年份:2015
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Development of specific inhibitors of Porphyromonas gingivalis gingipains based on their cognate propeptides
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21592372 - 财政年份:2009
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19791358 - 财政年份:2007
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Grant-in-Aid for Young Scientists (B)
Characterisation of the common steps in the glycosylation of Arg-gingipains and synthesis of LPS and APS of P.gingivalis
牙龈卟啉单胞菌精氨酸糖基化以及 LPS 和 APS 合成中常见步骤的表征
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Molecular characterization of the gingipains of Porphyromonas gingivalis
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nhmrc : 350427 - 财政年份:2005
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Examination of biological response to Porphyromonas gingivalis infection.
检查牙龈卟啉单胞菌感染的生物学反应。
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16591859 - 财政年份:2004
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