The Effect of antimicrobial agent to artificial biofilm formed by periodontopathic bacteria

抗菌剂对牙周病菌人工生物膜的影响

• 批准号: 17592164
• 负责人: NAKAGAWA Taneaki
• 金额: $ 2.01万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17592164/
• 关键词: periodontopathic bacteria; artificial biofilm; antimicrobial agent; 人口バイオフィルム

Oral biofilm is composed of many different microorganisms. An increase in pathogenic microorganisms which results in change of composition within the biofilm can cause dental caries and periodontal disease. In chronic periodontitis lesions, an increase in proportions of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia is frequently observed. The purpose of this study is to analyze the mechanism involved in biofilm formation, and evaluate anti-biofilm effects of antimicrobial agents in order to establish an effective periodontal treatment modality utilizing antimicrobial agents. Povidone-iodine showed higher bactericidal effects than chlorhexidine when effect for planktonic P. gingivalis was evaluated. Therefore we developed a model to quantitatively evaluate the effect of povidone-iodine on biofilm formation using a 24-well plate with cell culture inserts. The periodontopathic bacteria were inoculated on plycarbonate membrane in trypticase soy broth. Various concentrations of povidon-iodine were then added. The povidon-iodine gargle with concentrations of 2% or more showed 1, 000-fold increase in bactericidal effect on biofilm by P gingivalis compared with the control. A similar effect was observed on biofilm by F nucleatum, when the povidon-iodine was used with concentrations of 1% or more.

口腔生物膜由许多不同的微生物组成。病原微生物的增加导致生物膜内成分的变化，可能导致龋齿和牙周病。在慢性牙周炎病变中，经常观察到牙龈卟啉单胞菌、齿垢密螺旋体和连翘坦氏菌的比例增加。本研究的目的是分析生物膜形成的机制，并评估抗菌药物的抗生物膜作用，以建立有效的抗菌药物牙周治疗方式。当评估对浮游牙龈卟啉单胞菌的作用时，聚维酮碘显示出比洗必泰更高的杀菌效果。因此，我们开发了一个模型，使用带有细胞培养插管的 24 孔板定量评估聚维酮碘对生物膜形成的影响。将牙周病细菌接种在胰蛋白酶大豆肉汤中的聚碳酸酯膜上。然后添加不同浓度的聚维酮碘。与对照相比，浓度为 2% 或更高的聚维酮碘漱口液对牙龈卟啉单胞菌生物膜的杀菌效果增加了 1, 000 倍。当使用浓度为 1% 或更高的聚维酮碘时，具核梭菌对生物膜也观察到类似的效果。

项目成果

The antimicrobial effect of Povidone-Iodine(PVP-I)gargle to artificial biofilm

聚维酮碘(PVP-I)含漱液对人工生物膜的抑菌作用

• 发表时间: 2007
• 作者: Junya;Ota;Yasuo;Hosaka;Chie;Fukaya;Kazuyuki;Ishihara;Taneaki;Nakagawa
• 通讯作者: Nakagawa

Susceptibility of Actinobacillus actinomycetemcomitans to six antibiotics decreases as biofiim matures

随着生物膜的成熟，伴放线放线杆菌对六种抗生素的敏感性降低

• 发表时间: 2007
• 期刊: Journal of Antimicrobial Chemotherapy 59
• 作者: Naoko Takahashi;Kazuyuki Ishibara;et. al.
• 通讯作者: et. al.

The Efficacy of Povidone-Iodine Productsagainst Periodontopathic Bacteria

聚维酮碘产品对抗牙周病细菌的功效

• 发表时间: 2006
• 期刊: Dematology 212
• 作者: Taneaki Nakagawa;et al.
• 通讯作者: et al.

The antimicrobial effect of Povidone-iodine(PVP-I)gargle to periodontopathic bacteria

聚维酮碘（PVP-I）含漱液对牙周病菌的抗菌作用

• 发表时间: 2006
• 作者: Yasuo;Hosaka;Chie;Fukaya;Kazuyuki;Ishihara;Taneaki;Nakagawa
• 通讯作者: Nakagawa

Susceptibility of Actlnobacillus actinomycetemcomitans to six antibiotics decreases as biofilm matures

随着生物膜的成熟，伴放线放线杆菌对六种抗生素的敏感性降低

• 发表时间: 2007
• 期刊: Journal of Antimicrobial Chenotherapy 59
• 作者: Naoko Takahashi;Kazuyuki Ishihara;et. al.
• 通讯作者: et. al.