The Effect of antimicrobial agent to artificial biofilm formed by periodontopathic bacteria

抗菌剂对牙周病菌人工生物膜的影响

• 批准号: 17592164
• 负责人: NAKAGAWA Taneaki
• 金额: $ 2.01万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17592164/
• 关键词: periodontopathic bacteria; artificial biofilm; antimicrobial agent; 人口バイオフィルム

Oral biofilm is composed of many different microorganisms. An increase in pathogenic microorganisms which results in change of composition within the biofilm can cause dental caries and periodontal disease. In chronic periodontitis lesions, an increase in proportions of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia is frequently observed. The purpose of this study is to analyze the mechanism involved in biofilm formation, and evaluate anti-biofilm effects of antimicrobial agents in order to establish an effective periodontal treatment modality utilizing antimicrobial agents. Povidone-iodine showed higher bactericidal effects than chlorhexidine when effect for planktonic P. gingivalis was evaluated. Therefore we developed a model to quantitatively evaluate the effect of povidone-iodine on biofilm formation using a 24-well plate with cell culture inserts. The periodontopathic bacteria were inoculated on plycarbonate membrane in trypticase soy broth. Various concentrations of povidon-iodine were then added. The povidon-iodine gargle with concentrations of 2% or more showed 1, 000-fold increase in bactericidal effect on biofilm by P gingivalis compared with the control. A similar effect was observed on biofilm by F nucleatum, when the povidon-iodine was used with concentrations of 1% or more.

口服生物膜由许多不同的微生物组成。致病性微生物的增加，导致生物膜内的组成变化会导致龋齿和牙周疾病。在慢性牙周炎病变中，经常观察到牙龈卟啉单胞菌，treponema denticola和Tannerella forsythia的比例增加。这项研究的目的是分析与生物膜形成有关的机制，并评估抗菌剂的抗生物膜作用，以建立利用抗菌剂的有效牙周治疗方式。当评估浮游牙龈疟原虫的作用时，povidone-碘的杀菌作用比洗涤胺更高。因此，我们开发了一个模型，用于定量评估Povidone-碘对使用带有细胞培养物插入物的24孔板对生物膜形成的影响。牙周病细菌在胰蛋白酶大豆肉汤中接种了丙烯酸酯膜。然后加入各种浓度的Povidon-碘。与对照相比，浓度为2％或以上的Povidon-iodine摄入量为2％或以上，杀菌对生物膜的杀菌作用增加了1，000倍。当Povidon-iodine使用浓度为1％或以上时，F核对生物膜也观察到了类似的作用。

项目成果

The antimicrobial effect of Povidone-Iodine(PVP-I)gargle to artificial biofilm

聚维酮碘(PVP-I)含漱液对人工生物膜的抑菌作用

• 发表时间: 2007
• 作者: Junya;Ota;Yasuo;Hosaka;Chie;Fukaya;Kazuyuki;Ishihara;Taneaki;Nakagawa
• 通讯作者: Nakagawa

Susceptibility of Actinobacillus actinomycetemcomitans to six antibiotics decreases as biofiim matures

随着生物膜的成熟，伴放线放线杆菌对六种抗生素的敏感性降低

• 发表时间: 2007
• 期刊: Journal of Antimicrobial Chemotherapy 59
• 作者: Naoko Takahashi;Kazuyuki Ishibara;et. al.
• 通讯作者: et. al.

The antimicrobial effect of Povidone-iodine(PVP-I)gargle to periodontopathic bacteria

聚维酮碘（PVP-I）含漱液对牙周病菌的抗菌作用

• 发表时间: 2006
• 作者: Yasuo;Hosaka;Chie;Fukaya;Kazuyuki;Ishihara;Taneaki;Nakagawa
• 通讯作者: Nakagawa

The Efficacy of Povidone-Iodine Productsagainst Periodontopathic Bacteria

聚维酮碘产品对抗牙周病细菌的功效

• 发表时间: 2006
• 期刊: Dematology 212
• 作者: Taneaki Nakagawa;et al.
• 通讯作者: et al.

The Efficacy of Povidone-Iodine Products against Periodontopathic Bacteria

聚维酮碘产品对抗牙周病细菌的功效

• 发表时间: 2006
• 期刊: Dermatology 212
• 作者: Taneaki Nakagawa;et. al.
• 通讯作者: et. al.