The search of graded epigenetic change of oral cancer and Neighboring epithelial dysplasia

口腔癌及邻近上皮异型增生分级表观遗传改变的探索

• 批准号: 21592519
• 负责人: FUJITA Hajime
• 金额: $ 3万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592519/
• 关键词: 口腔扁平上皮癌; 異型上皮; エピジェネティクス; DNAメチル化; 多段階発癌

We obtained a tissue specimen of normal tissue(N), epithelial dysplasia(D) and cancer(C), and extracted genomic DNA from the sample of two carcinoma of mandibular gingiva. We analyzed DNA methylation using illumina HumanMethylation27 and conducted the statistical analysis of the results. The gene which a methylation level rose to progressively in N group→D group→C group was EPDR1, FOXL1, FLJ21159, HOXB4, PRAC, DAPK1, FLJ46156, WDR8, BARHL2, NFAM1. Conversely, the gene which a methylation level decreased progressively was MEGEA3, GJB7, MFAP2, GPLD1, LOC348645, MEGEA6, FCN2, CCL1, SLC24A2, C9orf84. For the cancellation of the individual difference, it will be necessary to increase cases in future.

我们获得了正常组织（N）、上皮异常增生（D）和癌（C）的组织标本，并从两个下颌牙龈癌样品中提取基因组DNA。我们使用Illumina HumanMethylation 27分析DNA甲基化，并对结果进行统计分析。N组→D组→C组甲基化水平进行性升高的基因为EPDR 1、FOXL 1、FLJ21159、HOXB 4、PRAC、DAPK 1、FLJ46156、WDR 8、BARHL 2、NFAM 1。相反，甲基化水平逐渐降低的基因是MEGEA3、GJB 7、MFAP 2、GPLD 1、LOC 348645、MEGEA6、FCN2、CCL 1、SLC 24 A2、C9orf84。为了取消个人差异，将来有必要增加病例。

项目成果

Tetraspanin gene expression levels as potential biomarkers for malignancy of gingival squamous cell carcinoma

• DOI: 10.1002/ijc.24297
• 发表时间: 2009-06-15
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Hirano, Chizuru;Nagata, Masaki;Takagi, Ritsuo
• 通讯作者: Takagi, Ritsuo

Assessment of 14 functional gene polymorphisms in Japanese patients with oral lichen planus: a pilot case-control study.

• DOI: 10.1016/j.ijom.2009.05.001
• 发表时间: 2009-09
• 期刊: International journal of oral and maxillofacial surgery
• 影响因子: 2.4
• 作者: H. Fujita;T. Kobayashi;H. Tai;M. Nagata;H. Hoshina;R. Nishizawa;R. Takagi;H. Yoshie

下顎骨エナメル上皮腫・腺様歯原性腫瘍ハイブリッド腫瘍の1例

下颌牙釉质上皮瘤与牙源性腺样体瘤混合瘤一例

• 发表时间: 2010
• 作者: Yoshiyuki Mori;Takafumi Susami;Daichi Chikazu;Hideto Saijo;Miyuki Sakiyama;Masako Matsuzaki;Masako Abe;Mamiko Wada;Mitsuyoshi Iino;and Tsuyoshi Takato;藤田一,池田順行,齋藤太郎,高木律男,林孝文,山崎学,朔敬
• 通讯作者: 藤田一,池田順行,齋藤太郎,高木律男,林孝文,山崎学,朔敬