Studies on the regulation of differentiation of the pluripotent dental pulp stem cells by non-coding RNA

非编码RNA调控多能牙髓干细胞分化的研究

• 批准号: 21592547
• 负责人: NOZAKI Tadashige
• 金额: $ 3万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592547/
• 关键词: 歯髄; 幹細胞; 多能性; 分化転換; 再生医学

To further evaluate the multipotency of dental pulp cells, and to investigate miRNA regulation of differentiation of these cells, we examined their in vitro differentiation into an endocrine cell lineage. After induction, insulin-producing cells were detected. Based on expression profiles by microRNA arrays, we identified targets, including molecules crucial for the differentiation into pancreatic beta cells. Thus, dental pulp cells have the potential to differentiate into an endocrine cell lineage by the regulation of miRNA following in vitro induction.

为了进一步评估牙髓细胞的多能性，并研究miRNA对这些细胞分化的调节作用，我们研究了牙髓细胞向内分泌细胞系的体外分化。诱导后，检测产生胰岛素的细胞。基于microRNA阵列的表达谱，我们确定了靶标，包括分化为胰腺细胞的关键分子。因此，在体外诱导下，牙髓细胞具有通过miRNA调控向内分泌细胞谱系分化的潜力。

项目成果

Analysis of gene expression levels of dental pulp cells during adipogenic differentiation

牙髓细胞成脂分化过程中基因表达水平分析

• 发表时间: 2011
• 作者: Nozaki T.;Ohura K.
• 通讯作者: Ohura K.

5-Aza-2′-deoxycytidine treatment induces skeletal myogenic differentiation of mouse dental pulp stem cells

• DOI: 10.1016/j.archoralbio.2010.03.003
• 发表时间: 2010-05-01
• 期刊: ARCHIVES OF ORAL BIOLOGY
• 影响因子: 3
• 作者: Nakatsuka, Ryusuke;Nozaki, Tadashige;Sonoda, Yoshiaki
• 通讯作者: Sonoda, Yoshiaki

miRNA expression profiles during differentiation of plurilpotent dental pulp cells

多能牙髓细胞分化过程中的miRNA表达谱

• 发表时间: 2010
• 作者: Nozaki T.;Ohura K.
• 通讯作者: Ohura K.

Gone expression profile of dental pulp cells during differentiation into an adipocyte lineage

牙髓细胞分化为脂肪细胞谱系期间的表达谱消失

• 发表时间: 2011
• 期刊: Journal of Pharmacological Sciences
• 影响因子: 3.5
• 作者: Nozaki;T.;Ohura;K
• 通讯作者: K

Gene Expression Profile of Dental Pulp Cells During Differentiation Into an Adipocyte Lineage

• DOI: 10.1254/jphs.10163fp
• 发表时间: 2011-03-01
• 期刊: JOURNAL OF PHARMACOLOGICAL SCIENCES
• 影响因子: 3.5
• 作者: Nozaki, Tadashige;Ohura, Kiyoshi
• 通讯作者: Ohura, Kiyoshi