Studies on the regulation of differentiation of the pluripotent dental pulp stem cells by non-coding RNA

为了进一步评估牙髓细胞的多能性，并研究miRNA对这些细胞分化的调控，我们研究了它们在体外分化为内分泌细胞谱系。诱导后，检测胰岛素产生细胞。基于microRNA阵列的表达谱，我们确定了靶点，包括分化为胰腺β细胞的关键分子。因此，牙髓细胞有潜力分化成内分泌细胞谱系的调控miRNA体外诱导。

To further evaluate the multipotency of dental pulp cells, and to investigate miRNA regulation of differentiation of these cells, we examined their in vitro differentiation into an endocrine cell lineage. After induction, insulin-producing cells were detected. Based on expression profiles by microRNA arrays, we identified targets, including molecules crucial for the differentiation into pancreatic beta cells. Thus, dental pulp cells have the potential to differentiate into an endocrine cell lineage by the regulation of miRNA following in vitro induction.