Evaluation of a mouse odontoma model and elucidation of the developmental mechanisms of odontoma

多聚ADP核糖聚合酶（Poly（ADP-ribose）polymerase，Parp 1）通过转录调控在细胞死亡和分化中发挥作用。在本研究中，对来自具有ICR/129 Sv遗传背景的Parp 1-/-和野生型Parp 1 ^<+/+>小鼠的脱钙下颌骨（包括门牙）进行组织学分析，所述小鼠维持基础饮食2年。共分析了来自雄性Parp 1 ^<-/->小鼠的10个样品和来自雄性Parp 1 ^<+/+>小鼠的13个样品。在所有来自Parp 1 ^<-/->小鼠的样本中，牙本质显示出显著的增殖，并且在牙髓腔内侧观察到不规则的成牙本质细胞。相比之下，来自Parp 1 ^<+/+>小鼠的样品中的成牙本质细胞在牙髓侧规则地排列，并且没有观察到釉质的异常。为探讨PARP 1在牙瘤发生发展中的作用，对7例复杂型和6例复合型牙瘤进行免疫组化分析， ...更多信息 PARP 1的表达。结果显示，PARP 1在牙胚样组织、减少的釉质上皮、未成熟牙本质和未成熟釉质中均有表达。此外，它的表达被检测到成牙本质细胞和相应的成釉细胞的上皮成分。这些发现表明PARP 1有助于构成牙瘤的细胞的分化。此外，PARP 1在不具有细胞核的鬼细胞的细胞质中表达。这些血影细胞的细胞质也被M30 CytoDEATH抗体强烈免疫染色，该抗体针对细胞角蛋白18在凋亡过程中产生的切割位点。由于鬼细胞被认为是代表一种类型的退行性细胞，积累硬角蛋白，和一些早期的凋亡事件似乎已经发生，这些观察表明，PARP 1的表达在鬼细胞经历凋亡有助于这些细胞的形成在牙肿瘤。少

Poly (ADP-ribose) polymerase (Parp1) is known to play roles in cell death and cell differentiation through transcriptional control. In the present study, decalcified mandibles, including the incisors, from Parp1-/- and wild-type Parp1^<+/+> mice with a genetic background of ICR/129Sv maintained on a basal diet for 2 years were subjected to histological analyses. A total of 10 samples from male Parp1^<-/-> mice and 13 samples from male Parp1^<+/+> mice were analyzed. In all samples from the Parp1^<-/-> mice, the dentin showed remarkable proliferation and irregularly lined odontoblasts were observed on the inside of the dental pulp cavity. In contrast, the odontoblasts in the samples from the Parp1^<+/+> mice were regularly lined up on the side of the dental pulp and no abnormalities of the enamel were observed. To elucidate the involvement of PARP1 in the development of human odontoma, samples obtained from 7 complex type and 6 compound type human odontoma cases were analyzed immunohist … More ochemically for their expressions of PARP1. The results revealed that PARP1 was expressed in tooth germ-like tissue, reduced enamel epithelium, immature dentin and immature enamel in the odontomas. Moreover, its expression was detected in odontoblasts and the epithelial component corresponding to ameloblasts. These findings indicate that PARP1 contributes to the differentiation of the cells that constitute odontomas. In addition, PARP1 was expressed in the cytoplasm of ghost cells that did not possess a nucleus. The cytoplasm of these ghost cells was also strongly immunostained by the M30CytoDEATH antibody raised against the cleavage site of cytokeratin 18 produced during the process of apoptosis. Since ghost cells are considered to represent a type of degenerative cells that accumulate hard keratins, and some early apoptosis events seem to have occurred, these observations indicate that PARP1 expression in ghost cells undergoing apoptosis contributed to the formation of these cells in odontomas. Less