Effect of Concomitant use of G-CSF in bone regeneration by CD34 positive cells.

到目前为止，在骨再生领域，我们已经使用了人工骨和自体骨。因此，存在手术侵入性、异物反应和空间保留等困难。在本研究中，虽然我们在开始时曾尝试使用来自外周血的CD34阳性干细胞来抑制方法的侵袭性，但我们可以从外周血中获得的细胞有限，无法在临床上完成该方法。因此，我们尝试使用人工骨、从骨髓和骨膜获得的干细胞的组合。因此，我们开展了有效的骨再生新方法。

So far in the field of bone regeneration, we have used artificial bone and autologous bone. Therefore, there are some difficulties, such as invasiveness of the surgery, foreign-body reaction and space reservation. In this study, at the beginning, although we had tried to use CD34 positive stem cells derived from peripheral blood to inhibit invasiveness of method, the cells which we could obtained from peripheral blood were limited to accomplish this method in clinic. Consequently, we have tried to use the combination of artificial bone, stem cells obtained from bone marrow and periosteum. So we carried out effective new method of bone regeneration.