Exhaustive analysis using DGGE method for oral flora causing the infective endocarditis

点击翻译按钮获取中文摘要

We established DGGE method based on the variety of the Rod shape-determining protein gene(rodA) as a target the oral streptococcus which was the maximal bacterial species in the oral cavity causing infective endocarditis(IE). Based on this, we performed exhaustive analysis of the bacterial flora as saliva specimen. Also, we estimated bacterial strain from an examination for biochemical property and a 16S rRNA gene of the strain from a patient who has contracted IE. We determined a draft sequence of the genomic DNA of strain identified with Streptococcus mutans and performed comparison between genomic sequence that was already presented on a database. then, we reviewed the pathogenesis factor that was specific for IE. We made the study of bacterial species associated with dental caries, a periodontal disease-causing bacteria, the investigation of infecting organism for opportunistic infections by using an examination for dental caries, an examination for periodontal disease, fence-sitting bacteria examination of BML company.