Exhaustive analysis using DGGE method for oral flora causing the infective endocarditis

利用DGGE法对引起感染性心内膜炎的口腔菌群进行详尽分析

• 批准号: 21592602
• 负责人: NISHIGUCHI Miyuki
• 金额: $ 2.91万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592602/
• 关键词: 感染性心内膜炎; DGGE法; 口腔フローラ; 微生物群集解析; rodA遺伝子; ゲノムシークエンス; 歯科治療

We established DGGE method based on the variety of the Rod shape-determining protein gene(rodA) as a target the oral streptococcus which was the maximal bacterial species in the oral cavity causing infective endocarditis(IE). Based on this, we performed exhaustive analysis of the bacterial flora as saliva specimen. Also, we estimated bacterial strain from an examination for biochemical property and a 16S rRNA gene of the strain from a patient who has contracted IE. We determined a draft sequence of the genomic DNA of strain identified with Streptococcus mutans and performed comparison between genomic sequence that was already presented on a database. then, we reviewed the pathogenesis factor that was specific for IE. We made the study of bacterial species associated with dental caries, a periodontal disease-causing bacteria, the investigation of infecting organism for opportunistic infections by using an examination for dental caries, an examination for periodontal disease, fence-sitting bacteria examination of BML company.

我们建立了基于棒状决定蛋白基因（rodA）变异的DGGE方法，以口腔内引起感染性心内膜炎（IE）的最大细菌种类口腔链球菌为靶点。在此基础上，我们对唾液样本的细菌菌群进行了详尽的分析。此外，我们通过检查生化特性和从感染IE的患者身上提取的菌株的16S rRNA基因来估计菌株。我们确定了突变链球菌菌株基因组DNA的草图序列，并与数据库中已经存在的基因组序列进行了比较。然后，我们回顾了IE特有的发病因素。我们利用BML公司的龋病检查、牙周病检查、坐位细菌检查，对与龋病相关的细菌种类进行了研究，并对机会性感染的感染微生物进行了调查。

项目成果

Phylogenetic analyses and detection of viridans streptococci based on sequences and denaturing gradient gel electrophoresis of the rod shape-determining protein gene.

• DOI: 10.3402/jom.v1i0.2015
• 发表时间: 2009-08-28
• 期刊: Journal of oral microbiology
• 影响因子: 4.5
• 作者: Konishi, Ikuri;Hoshino, Tomonori;Fujiwara, Taku
• 通讯作者: Fujiwara, Taku

Phylogenetic analyses and detection of viridans streptococci based on sequences and denaturing gradiend gel electrophoresis of the rodshaped-determining protein gene

基于杆状决定蛋白基因序列和变性梯度凝胶电泳的草绿色链球菌系统发育分析和检测

• 发表时间: 2009
• 期刊: Jounal of Oral Microbiology
• 作者: Konishi I;Hoshino T;Kondo Y;Saito K Nishiguchi M;Sato K;Fujiwara T
• 通讯作者: Fujiwara T