A study of the clinical bacteriology of the infective endocarditis in the pedodontics by using molecular technique

1.牙科治疗与感染性心内膜炎（IE）相关性的问卷调查：我们对800家日本儿科学会授权的一位专家进行问卷调查，以阐明牙科治疗与IE的相关性。结果，儿科医生继续对IE高度感兴趣，并倾向于执行盲目抗菌药物的预防，而与先天性心脏病儿童的出血牙科治疗风险的数量无关。本调查中IE发病率为2.7%，不认为是牙科治疗所致。然而;发病例显示抗菌药物预防剂量略低的趋势。微生物群体分析方法的建立，确定牙齿IE的治疗和原因：作为确定牙齿IE的治疗和原因的手段，我们开发了微生物群体的DGGE分析方法，采用棒形决定蛋白基因（rodA）基因。首先在人口腔中选取所有检出可能性高的27株口腔链球菌型菌株，采用PCR扩增rodA基因。直接序列是用和PCR中使用的引物一样的东西来扩增产物，我们确定序列。接下来，我们对获得的链球菌型菌株的rodA基因进行遗传系统分析，根据GC含量计算Tm值，并确定DNA的变性条件（尿素密度、迁移温度），每个菌株都可以用DGGE法检测到不同的迁移模式。在此条件下，我们对所有链球菌型菌株27株进行了rodA基因扩增切片电泳，并对每个菌株的迁移模式进行了分析。因此，通过将直接从唾液中提取的细菌DNA扩增到模板的基因片段的迁移模式与上述型菌株的迁移模式进行比较，可以对唾液中的微生物群体进行分析。少

1.Dental treatment and questionary survey about the association of the infective endocarditis(IE) :We performed questionary survey for a specialist in 800 Japanese Society of Pedodontics authorizations to elucidate dental treatment and association of IE. As a result the pedodontist continued having interest for IE highly, and a tendency to perform the prophylaxis of the blind antimicrobial agent was suggested without being associated with the number of the risk for the bloody dental treatment of the child of the congenital heart disease. The onset of IE was 2.7% in this investigation, and it was not thought that it was caused by dentistry treatment. However; the tendency that the prophylactic dosage of the antimicrobial agent was low quantity slightly was suggested in the onset example.2. The establishment of the microbe crowd analytical method to determine dental treatment and causation of IE :As means to determine dental treatment and causation of IE, we developed the microbe crowd a … More nalytical method by DGGE method using a rod shape determining protein gene (rodA)gene. At first we chose all high oral cavity streptococcal type strain 27 stocks of detected possibility among human oral cavity and amplified a rodA gene by PCR method. A direct sequence did an amplified product using a thing same as the primer which we used in PCR, and we determined the sequence. Next time, we analyzed a heredity system about the rodA gene of obtained streptococus type strain and calculated Tm value from the GC content and determined a denaturation condition (the density of urea, migration temperature)of the DNA which a difference could detect in a migration pattern every each strain of can put it in DGGE method. We electrophoresed a rodA gene amplification section of all streptococus type strain 27 stocks under this condition and did profiling of a migration pattern every strain. Therefore, the analysis of the microbial crowd of saliva was enabled by comparing the migration pattern of the gene section that amplified the bacteria DNA which it extracted from saliva directly to a template and a migration pattern of the type strain mentioned above. Less