Establishment of artificial synthesis of Nano bacteria and dental calculus

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We tried to purify the proteins that react to the monoclonal antibody for the Nano bacteria from the bovine serum. These proteins strongly bind to the hydroxyapatite. Among these proteins, one of them binds to hydroxyapatite by cationic reactions and others are bind to hydroxyapatite by affinity reactions for the calcium. We success in the purification of the protein that bind to hydroxyapatite by cationic reactions. By the gel filtration chromatography, other proteins that bind to the hydroxyapatite by affinity reactions for the calcium were collected in fixed fractions, however, we could not single out these proteins. By the mass analysis, these proteins included the components of the common fatty acid complexes that related to the life style diseases.