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The Basic research on the probiotics for the prevention of the periodontal disease and halitosis.

益生菌预防牙周病和口臭的基础研究。

基本信息

批准号：
15592219
负责人：
NOMURA Yoshiaki
金额：
$ 2.18万
依托单位：
Tsurumi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

By the in-vitro investigation, the growth of the Porphyromonas gingivalis was suppressed by the co-cultivation with Lactobacillus or Enterococcus faecali (E.faecalis). However, this may be due to the lowering pH accompanied by the cultivation of these bacteria. In the oral cavity, it is hard to consider these bacteria control the pH of the some of the oral fluid. The effect of probiotics should be considerd by the another aspects. Thus, we co-cultivate Streptococcus mutans (S.mutans) MT8148,OMZ175 or Streptococcus sobrinus (S.sobrinus) 6715, 33478 and L.casei IAM12473 Streptococcus mitis (S.mitis) 6249, Enterococcus faecalis (E.faecalis) BIO 3B by several proportions of these bacteria. By the co-cultivation, the production of the water insoluble glucans produced by the S.mutans and S.sobrinus were suppressed according to the dose response manner of the proportion. However, the growth of the S.mutans and S.sobrinus were not suppressed by the co-cultivation. In addition, the co-cultivation with E.faecalis the colony forming of the S.mutans and S.sobrinus were inhibited. According to these results, we concluded that the effect of the probiotics for the periodontal disease or halitosis may be derived form the indirect effect such as inhibition of the producing the biofilms rather than direct effect to suppress the growth of the periodontal bacteria.

通过体外实验研究，牙龈卟啉单胞菌与乳酸杆菌或粪肠球菌（E.faecalis）共培养可抑制牙龈卟啉单胞菌生长。然而，这可能是由于这些细菌的培养伴随着pH值的降低。在口腔中，很难认为这些细菌控制了一些口腔液的pH值。益生菌的作用还有待于其他方面的研究。因此，我们通过几种比例的这些细菌共培养变形链球菌（S.mutans）MT 8148、OMZ 175或远缘链球菌（S.sobrinus）6715、33478和干酪乳杆菌IAM 12473缓症链球菌（S.mitis）6249、粪肠球菌（E.faecalis）BIO 3B。通过共培养，变形链球菌和远缘链球菌产生的水不溶性葡聚糖的产量根据比例的剂量反应方式被抑制。然而，变形链球菌和远缘链球菌的生长并没有受到共培养的抑制。此外，与粪肠球菌共培养对变形链球菌和远缘链球菌的菌落形成有抑制作用。根据这些结果，我们得出结论，益生菌对牙周病或口臭的作用可能是通过间接作用，如抑制生物膜的产生，而不是直接作用于抑制牙周细菌的生长。