Analysis of migraine pathophysiology in an animal model using small-animal PET

使用小动物 PET 分析动物模型偏头痛病理生理学

• 批准号: 21600020
• 负责人: CUI Yilong
• 金额: $ 2.75万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21600020/
• 关键词: PET; 片頭痛; 痛み; 脳内炎症; 神経活動; 脳機能画像解析; 疼痛; PETイメージング; 脳機能解剖学; 神経回路

Using in vivo PET imaging with FDG and[^<11> C] PK11195, a PET ligand for translocator protein(TSPO, previously called peripheral benzodiazepine receptor), we evaluated the pain matrix and neurogenic inflammatory process in the rat brain after generation of cortical spreading depression(SD), a stimulation used for making an experimental animal model of migraine. The results show that the uptake of[^<11> C] PK11195, which has been used extensively to image activated microglia in the central nervous system, in the unilateral SD model rats increased in the ipsilateral hemisphere, and completely displaced by excess unlabeled ligands. Moreover, a quantitative analysis showed the binding potential for[^<11> C] PK11195 to significantly increase in the ipsilateral hemisphere, suggesting a pathological role of microglia activation in migraine etiology. The results further show that the regional brain activity was significantly increased in several brain regions, including the ipsilateral brainstem, contralateral sensory, and insular cortex, which have been reported to be involved in a nociceptive pathway in migraine.

使用FDG的活体PET成像和转位蛋白(TSPO，以前称为外周苯二氮卓类受体)的PET配体[^&lt；11&gt；C]PK11195，我们评估了皮质扩散性抑制(SD)后大鼠大脑中的疼痛基质和神经源性炎症过程，SD是一种用于制造偏头痛实验动物模型的刺激。结果表明，广泛用于中枢神经系统小胶质细胞成像的PK11195在单侧SD模型大鼠同侧大脑半球摄取增加，并被过量的未标记配体完全取代。此外，定量分析显示，[^&lt；11&gt；C]PK11195在同侧大脑半球的结合潜力显著增加，提示小胶质细胞激活在偏头痛病因中的病理作用。结果进一步表明，在偏头痛的几个脑区，包括同侧脑干、对侧感觉和岛叶皮质，局部脑活动显著增加，据报道，这些区域参与了偏头痛的伤害性通路。

项目成果

Stoichiometry-focused 18F-labeling of alkyne-substituted oligodeoxynucleotides using azido([18F]fluoromethyl)benzenes by Cu-catalyzed Huisgen reaction

• DOI: 10.1016/j.bmc.2010.11.033
• 发表时间: 2011-01-01
• 期刊: BIOORGANIC & MEDICINAL CHEMISTRY
• 影响因子: 3.5
• 作者: Kuboyama, Takeshi;Nakahara, Motoi;Doi, Hisashi
• 通讯作者: Doi, Hisashi

MicroPETを用いたラット脳内の疼痛認知・伝達回路の解析

使用 MicroPET 分析大鼠大脑中的疼痛感知和传输回路

• 发表时间: 2011
• 作者: Cui;Y. L;崔翼龍,佐古健生,奥山香里,豊田浩士,尾上嘉代,林中恵美,和田康弘,渡辺恭良,片岡洋祐
• 通讯作者: 崔翼龍,佐古健生,奥山香里,豊田浩士,尾上嘉代,林中恵美,和田康弘,渡辺恭良,片岡洋祐

tron emission tomography (PET) imaging-based evaluation of hepatobiliary transport in humans using 15R-[^<11>C]TIC-Me

使用 15R-[^<11>C]TIC-Me 对人类肝胆运输进行基于 tron 发射断层扫描 (PET) 成像的评估

• 期刊: J Nucl Med
• 影响因子: 9.3
• 作者: Takashima;T.;Kitamura;S.;Wada;Y.;Tanaka;M.;Shigihara;Y.;Ishii;H.;Ijuin;R.;Shiomi;S.;Nakae;T.;Watanabe;Y.;Cui;Y.L.;Doi;H.;Suzuki;M.;Maeda;K.;Kusuhara;H.;Sugiyama;Y.;Watanabe;Y.
• 通讯作者: Y.

片頭痛病態モデルラットを用いた疼痛伝達回路の解析

偏头痛模型大鼠的疼痛传递回路分析

• 发表时间: 2010
• 作者: Akazawa;K. H.;Cui;Y. L.;Tanaka;M.;Kataoka;Y.;Yoneda;Y.;and Watanabe;Y;原英子;崔翼龍,佐古健生,豊田浩士,奥山香里,尾上嘉代,林中恵美,和田康弘,渡辺恭良,片岡洋祐
• 通讯作者: 崔翼龍,佐古健生,豊田浩士,奥山香里,尾上嘉代,林中恵美,和田康弘,渡辺恭良,片岡洋祐

11C-PK11195 PET for the In Vivo Evaluation of Neuroinflammation in the Rat Brain After Cortical Spreading Depression

• DOI: 10.2967/jnumed.109.066498
• 发表时间: 2009-11-01
• 期刊: JOURNAL OF NUCLEAR MEDICINE
• 影响因子: 9.3
• 作者: Cui, Yilong;Takashima, Tadayuki;Watanabe, Yasuyoshi
• 通讯作者: Watanabe, Yasuyoshi