Clarification of Fermentative Metabolism Flow of Ethanol-fermenting Fungi and Challenge to Consolidated Biopreocessing for Bioethanol Production

发酵乙醇真菌爪哇毛霉在好氧条件下具有较强的戊糖发酵乙醇能力。为了阐明这一现象和戊糖的代谢途径，通过CE-TOFMS代谢组分析确定了从木糖到乙醇的代谢流程。为了提高菌种对纤维素酶的分泌反应，从毛霉文库中筛选出一株分泌内切β-葡聚糖酶能力强的真菌，并构建了该真菌与爪哇分枝杆菌J的融合细胞。将制备的稻草融合细胞与CBP进行碱化处理，可直接生产5.2g/L的乙醇。

Ethanol-fermenting fungi Mucor javanicus J has strong ethanol fermentation ability from pentose under aerobic condition. To clarify this phenomenon and the pentose metabolic pathway, the metabolism flow from xylose to ethanol was ascertained by the metabolome analysis by CE-TOFMS. To improve the cellulolytic enzyme secretory response of the bacterium share, The fungus with high ability to secrete endo-β-glucanase was screened among our Mucor library and then the fusion cell of the fungus and M. javanicus J was constructed. When CBP with the fusion cell from rice straw prepared alkaline treatment were carried out, 5. 2 g/L of ethanol was able to directly produce.